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Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects

2003-01-24Journal article Restricted access
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dc.contributor.authorFerreira, Bruno S.
dc.contributor.authorCalado, Cecília
dc.contributor.authorKeulen, Frederik van
dc.contributor.authorFonseca, Luís P. P.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorR., Da Fonseca M. M.
dc.date.accessioned2020-10-01T16:47:25Z
dc.date.available2020-10-01T16:47:25Z
dc.date.issued2003-01-24
dc.description.abstractAlthough the physiology and metabolism of the growth of yeast strains has been extensively studied, many questions remain unanswered where the induced production of a recombinant protein is concerned. This work addresses the production of a Fusarium solani pisi cutinase by a recombinant Saccharomyces cerevisiae strain induced through the use of a galactose promoter. The strain is able to metabolise the inducer, galactose, which is a much more expensive carbon source than glucose. Both the transport of galactose into the cell—required for the induction of cutinase production—and galactose metabolism are highly repressed by glucose. Different fermentation strategies were tested and the culture behaviour was interpreted in view of the strain metabolism and physiology. A fed-batch fermentation with a mixed feed of glucose and galactose was carried out, during which simultaneous consumption of both hexoses was achieved, as long as the glucose concentration in the medium did not exceed 0.20 g/l. The costs, in terms of hexoses, incurred with this fermentation strategy were reduced to 23% of those resulting from a fermentation carried out using a more conventional strategy, namely a fed-batch fermentation with a feed of galactose.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFERREIRA, Bruno S.; [et al] – Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects. Applied Microbiology and Biotechnology. ISSN 1432-0614. Vol. 61, N.º 1 (2003), pp. 69–76pt_PT
dc.identifier.doi10.1007/s00253-002-1196-0pt_PT
dc.identifier.issn1432-0614
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10400.21/12267
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationPRAXIS XXI programme (grant GGP XXI/BD/2936/96)pt_PT
dc.relationPRAXIS XXI programme (grant GGP XXI/BD/ 18276/98)pt_PT
dc.subjectFusarium solani pisi cutinasept_PT
dc.subjectRecombinant Saccharomyces cerevisiaept_PT
dc.subjectGalactose promoterpt_PT
dc.titleTowards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspectspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage76pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage69pt_PT
oaire.citation.titleApplied Microbiology and Biotechnologypt_PT
oaire.citation.volume61pt_PT
person.familyNameCalado
person.familyNameP. Fonseca
person.familyNameCabral
person.familyNameda Fonseca
person.givenNameCecília
person.givenNameLuis
person.givenNameJoaquim M.S.
person.givenNameMaria Manuela
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id951D-DF38-3397
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0001-8429-6977
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0002-1498-6195
person.identifier.ridE-2102-2014
person.identifier.ridA-4228-2013
person.identifier.ridG-2052-2010
person.identifier.ridI-1321-2012
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id55916288400
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id7005461168
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication9bfc3a59-5577-4b4b-aaa0-7875d468d981
relation.isAuthorOfPublication.latestForDiscovery9bfc3a59-5577-4b4b-aaa0-7875d468d981

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