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Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases

2012-11Journal article Restricted access
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dc.contributor.authorRosado, Tânia
dc.contributor.authorBernardo, Pedro
dc.contributor.authorKoci, Kamila
dc.contributor.authorCoelho, Ana V.
dc.contributor.authorRobalo, Maria Paula
dc.contributor.authorMartins, Lígia O.
dc.date.accessioned2015-09-08T14:50:13Z
dc.date.available2015-09-08T14:50:13Z
dc.date.issued2012-11
dc.description.abstractThe aim of the present work is to provide insight into the mechanism of laccase reactions using syringyl-type mediators. We studied the pH dependence and the kinetics of oxidation of syringyl-type phenolics using the low CotA and the high redox potential TvL laccases. Additionally, the efficiency of these compounds as redox mediators for the oxidation of non-phenolic lignin units was tested at different pH values and increasing mediator/non-phenolic ratios. Finally, the intermediates and products of reactions were identified by LC-MS and H-1 NMR. These approaches allow concluding on the (1) mechanism involved in the oxidation of phenolics by bacterial laccases, (2) importance of the chemical nature and properties of phenolic mediators, (3) apparent independence of the enzyme's properties on the yields of non-phenolics conversion, (4) competitive routes involved in the catalytic cycle of the laccase-mediator system with several new C-O coupling type structures being proposed.por
dc.identifier.citationROSADO, T.; [et al] – Methyl syringate: Na efficient phenolic mediator for bacterial and fungal laccases. Bioresource Technology. ISSN: 0960-8524. Vol. 124 (2012), pp. 371-378por
dc.identifier.doi10.1016/j.biortech.2012.08.023
dc.identifier.issn0960-8524
dc.identifier.urihttp://hdl.handle.net/10400.21/5110
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Sci LTDpor
dc.relationEuropean Commission - IORENEW-FP6-2004-NMP-NI-4/026456
dc.relationFundação para a Ciência e Tecnologia (FCT), Portugal - PTDC/AGR-CFL/103840/2008
dc.relationFundação para a Ciência e Tecnologia (FCT), Portugal - PEst-OE/EQB/LA0004/2011
dc.relationFCT, Portugal - SFRH/BD/65747/2009
dc.relationFCT, Portugal - REDE-1504
dc.relationEC - BIORENEWFP6-2004-NMP-NI-4/026456
dc.relationEC - PTDC/ AGR-CFL/103840/2008
dc.relationFCT - PEst-OE/EQB/LA0004/2011
dc.relationREDE/1504/RNEM/2005
dc.relationFCT - SFRH/BD/65747/2009
dc.relationFCT - REDE-1504
dc.subjectLaccasespor
dc.subjectPhenolicspor
dc.subjectNatural Mediatorspor
dc.subjectNon-Phenolicpor
dc.subjectLignin Unitspor
dc.subjectRadical Couplingpor
dc.subjectNatural Mediators
dc.subjectCrystal-Structure
dc.subjectTrametes-Versicolor
dc.subjectEndospore Coat
dc.subjectPaper Pulp
dc.subjectOxidation
dc.subjectLignin
dc.subjectComponent
dc.subjectSystems
dc.subjectFlax
dc.titleMethyl syringate: An efficient phenolic mediator for bacterial and fungal laccasespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceOxon
oaire.citation.endPage378por
oaire.citation.startPage371por
oaire.citation.titleBioresource Technologypor
oaire.citation.volume124por
person.familyNameRobalo
person.familyNameMartins
person.givenNameMaria Paula
person.givenNameLígia O.
person.identifier319225
person.identifier.ciencia-idE11C-A704-9C18
person.identifier.ciencia-id731B-B843-C1DF
person.identifier.orcid0000-0002-8200-6910
person.identifier.orcid0000-0003-0082-9591
person.identifier.ridB-2002-2012
person.identifier.ridC-2513-2009
person.identifier.scopus-author-id9269662100
person.identifier.scopus-author-id7102109314
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication823a9498-17ab-451f-bb8f-56338643ac0c
relation.isAuthorOfPublicationcaca2712-516a-4db1-83a5-9309ba28954b
relation.isAuthorOfPublication.latestForDiscovery823a9498-17ab-451f-bb8f-56338643ac0c

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