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Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium

2020-08Journal article Restricted access
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dc.contributor.authorFadel, Sezan R.
dc.contributor.authorBendif, Hamdi
dc.contributor.authorGuedes, Laura
dc.contributor.authorAndré, Rebeca
dc.contributor.authorPacheco, Rita
dc.contributor.authorGuedes, Rita
dc.contributor.authorMerabti, Karim
dc.contributor.authorMiara, Mohamed Djamel
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2021-01-19T16:33:12Z
dc.date.available2021-01-19T16:33:12Z
dc.date.issued2020-08
dc.description.abstractIntroduction: Verbascum betonicifolium (V. betonicifolium) is a plant used in traditional medicine for several ailments. The objective of this study was to determine the antioxidant activity and acetylcholinesterase inhibitory activity of the aqueous extract together with the metabolites responsible for these activities. This paper presents the first phytochemical characterization and bioactivities of aqueous extracts from aerial parts. Methods: The compounds present in the aerial part aqueous extract were identified by high performance liquid chromatography, coupled to a diode-array detector (HPLC-DAD) and by high-resolution mass spectrometry (HRMS), using LC-MS/MS analyses. Antioxidant activity was measured as the capacity to scavenge the free radical DPPH and the AChE activity was determined using the Ellman test. The cytotoxicity was determined used HepG2 cell lines. Results: Several types of metabolites were found, primary metabolites (malic, citric and gluconic acids), phenolic compounds (verbascoside, luteolin), terpenoids (iridoid glycoside, unedide), among others. The total phenol content of 28 mu g of gallic acid equivalents/mg of extract was determined. The aqueous extract antioxidant activity had and EC50 of 70 mu g/mL and the AChE inhibitory activity an IC50 of 750 mu g/mL. No cytotoxicity towards HepG2 cells was detected, even using a concentration of 1 mg/mL. Conclusions: The phenolic compounds present in the extract may be the main contributors to the bioactivities of V. betonicifolium. These results show for the first time the richness of phytochemicals and the strong bioactivities of V. betonicifolium and that the aqueous extract could be used as new natural sources of bioactive molecules.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFADEL, Sezan R.; [et al] – Bioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifolium. European Journal of Integrative Medicine. ISSN 1876-3820. Vol. 37 (2020), pp. 1-7pt_PT
dc.identifier.doi10.1016/j.eujim.2020.101171pt_PT
dc.identifier.eissn1876-3839
dc.identifier.issn1876-3820
dc.identifier.urihttp://hdl.handle.net/10400.21/12652
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUIDB/04046/2020 - FCTpt_PT
dc.relationUIDP/04046/2020 - FCTpt_PT
dc.relationPTDC/BIA-BQM/28355/2017 - FCTpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S1876382020308519?token=09DA834D62F76784DA38E09EB600F9360BD62A751E04B7AE6753D6563D29D2CB34629BF47A8D3D20AD72F8BD69A5F06Dpt_PT
dc.subjectVerbascum betonicifoliumpt_PT
dc.subjectHRMSpt_PT
dc.subjectPhytochemical compositionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectAcetylcholinesterase inhibitory activity;pt_PT
dc.subjectCytotoxicitypt_PT
dc.titleBioactivities of iridoids and flavonoids present in decoctions from aerial parts of Verbascum betonicifoliumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleEuropean Journal of Integrative Medicinept_PT
oaire.citation.volume37pt_PT
person.familyNameAndré
person.familyNamePacheco
person.familyNameGuedes
person.familyNameSerralheiro
person.givenNameRebeca
person.givenNameRita
person.givenNameRita
person.givenNameMaria Luisa
person.identifier1173301
person.identifier1022927
person.identifier2742791
person.identifier.ciencia-id7D18-8206-8EF7
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-id5F1E-E3BE-991C
person.identifier.orcid0000-0002-2733-5797
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-5488-9042
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridAGX-2106-2022
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id57207778987
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6602688573
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery25c176b0-79c0-4a9c-9bbd-92d6b5dee1c2

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