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Antigenic and conserved peptides from diverse Helicobacter pylori antigens

2022-03-11Journal article Open access
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dc.contributor.authorCalado, Cecília
dc.date.accessioned2022-03-15T10:12:08Z
dc.date.available2022-03-15T10:12:08Z
dc.date.issued2022-03-11
dc.description.abstractSince the revolutionary fnding of Helicobacter pylori as a common bacterial infection, that a high research efort for its eradication has been conducted. Epitope based-vaccine presents advantages over protein-based, as they can be designed to contain epitopes from diverse proteins, therefore, more easily representing the immune-variability of the bacterial population, while minimizing the toxicity associated to some whole proteins. In the present work, an iterative method, to design antigenic and conserved B-epitopes from diverse virulent factors of H. pylori, was established. The method considered the trade-of between epitopes antigenicity and conservation among the bacterial population. For the method validation, fve virulent factors from H. pylori were selected. From each virulent factor, two epitopes were predicted, each with twelve residues of aminoacids. The corresponding ten peptides were synthesised and evaluated by enzyme-linked immunosorbent assay using polyclonal antibodies raised against a specifc H. pylori strain. All ten peptides were recognised by the antibodies and were consequently antigenic and conserved. This result could strongly contribute to the design of a multivalent epitope-based vaccine, representing the immunogenetic variability within the bacterial population, leading to a sustained and efective immunogenic protection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecília R. C. – Antigenic and conserved peptides from diverse Helicobacter pylori antigens. Biotechnology Letters. ISSN 0141-5492. Vol. 44, N.º 3 (2022), pp. 535-545.pt_PT
dc.identifier.doi10.1007/s10529-022-03238-xpt_PT
dc.identifier.eissn1573-6776
dc.identifier.issn0141-5492
dc.identifier.urihttp://hdl.handle.net/10400.21/14464
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/content/pdf/10.1007/s10529-022-03238-x.pdfpt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectVaccinept_PT
dc.subjectEpitopespt_PT
dc.subjectMulti-antigenspt_PT
dc.subjectPeptidept_PT
dc.titleAntigenic and conserved peptides from diverse Helicobacter pylori antigenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.citation.endPage545pt_PT
oaire.citation.issue3
oaire.citation.startPage535pt_PT
oaire.citation.titleBiotechnology Letterspt_PT
oaire.citation.volume44
oaire.fundingStream3599-PPCDT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicatione934d4a3-07d0-4c5d-8183-e76b3b0bf237
relation.isProjectOfPublication.latestForDiscoverye934d4a3-07d0-4c5d-8183-e76b3b0bf237

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