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Effect of pre-fermentation on the production of cutinase by a recombinant Saccharomyces cerevisiae

2002-04-04Journal article Restricted access
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dc.contributor.authorCalado, Cecília
dc.contributor.authorMonteiro, Sandra M. S.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFonseca, Luís P. P.
dc.date.accessioned2020-10-06T14:43:32Z
dc.date.available2020-10-06T14:43:32Z
dc.date.issued2002-04-04
dc.description.abstractThe importance of controlling the expression of heterologous cutinase in a recombinant Saccharomyces cerevisiae SU50 strain was investigated. Maximum specific growth rate and the biomass yield increased 1.91 and 1.16 fold, respectively, when cutinase production was induced by galactose in a pre-fermentation step. However, only 19% of specific cell activity was obtained in comparison to other fermentations following a pre-fermentation step without induction of cutinase expression. Thus, the pre-fermentation step was performed using a selective medium not containing galactose, and the fermentation was performed with a cheaper and complex non-selective medium containing galactose. Under these conditions, and with the aim of maximising the specific cutinase activity, a pre-fermentation with low volume and high density of viable cells must be used. However, due to the low pre-fermentation volume, low yeast cell concentrations and low specific cell activities were obtained after 96 h of fermentation. Otherwise, when the aim was to maximise cutinase yield and productivity, a pre-fermentation volume of 10% (v/v) in relation to fermentation and in the exponential growth phase with a cell concentration between 1.1 and 1.8 g dcw/l should be used. A higher pre-fermentation volume, such as 20% (v/v), would still be economical in the case of a pre-fermentation with low cell density or low cell viability.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecília R. C.; [et al] – Effect of pre-fermentation on the production of cutinase by a recombinant Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering. ISSN 1389-1723. Vol. 93, N.º 4 (2002), pp. 354-359pt_PT
dc.identifier.doi10.1016/s1389-1723(02)80067-6pt_PT
dc.identifier.issn1389-1723
dc.identifier.urihttp://hdl.handle.net/10400.21/12274
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRAXIS XXI, Ministério da Ciência e Tecnologia, Portugal (BD/18276/98)pt_PT
dc.relationEU Project: Integrated Bioprocess Design for Large Scale Production and Isolation of Recombinant Proteins (BIO4-CT96-0435)pt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S1389172302800676?token=C645B59591C951F96A743942BCE0342CBA72824160616BDA9857ABE78FFA2AB504C235AF3F11D6F28528A5CFC0AB8AE6pt_PT
dc.subjectCutinasept_PT
dc.subjectFusarium solani pisipt_PT
dc.subjectInoculumpt_PT
dc.subjectPre-fermentationpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleEffect of pre-fermentation on the production of cutinase by a recombinant Saccharomyces cerevisiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage359pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage354pt_PT
oaire.citation.titleJournal of Bioscience and Bioengineeringpt_PT
oaire.citation.volume93pt_PT
person.familyNameCalado
person.familyNameCabral
person.familyNameP. Fonseca
person.givenNameCecília
person.givenNameJoaquim M.S.
person.givenNameLuis
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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