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Monitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli cultures

2011-09-23Conference object Open access
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dc.contributor.authorScholz, Teresa
dc.contributor.authorLopes, Vítor V.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2020-07-31T12:26:06Z
dc.date.available2020-07-31T12:26:06Z
dc.date.issued2011-09-23
dc.description.abstractFourier transform infrared (FTIR) spectroscopy is evaluated as a tool to monitor two bacterial processes: strain discrimination and drug inactivation studies with the gastric pathogen Helicobacter pylori and the plasmid production process based on high-density cultures of recombinant Escherichia coli. Results show, that after evaluation of different incubation conditions of H.pylori with the drug model, the application of principal component analysis to the FTIR spectra assembles the samples into clusters which can be related with the minimal inhibitory concentration. Morever, the same methodology applied to FTIR spectra from 12 different strains can be used to distinguish them. For the E.coli cultures it is possible to estimate the concentration of relevant bioprocess monitoring variables, such as plasmid, biomass, and carbon sources (glucose, glycerol, acetate) by using partial least squares (PLS) models based on FTIR spectra.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSCHOLZ, Teresa; LOPES, Vítor V.; CALADO, Cecília R. C. – Monitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli cultures. In 1st Portuguese Biomedical Engineering Meeting. Lisbon, Portugal: IEEE, 2011. ISBN 978-1-4577-0522-9. Pp. 1-6pt_PT
dc.identifier.doi10.1109/ENBENG.2011.6026090pt_PT
dc.identifier.isbn978-1-4577-0522-9
dc.identifier.isbn978-1-4577-0523-6
dc.identifier.isbn978-1-4577-0521-2
dc.identifier.urihttp://hdl.handle.net/10400.21/12129
dc.language.isoengpt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6026090pt_PT
dc.subjectFTIR spectroscopypt_PT
dc.subjectChemometricspt_PT
dc.subjectH.pyloript_PT
dc.subjectE.colipt_PT
dc.subjectDrug inactivationpt_PT
dc.subjectBioprocess monitoringpt_PT
dc.titleMonitoring bacterial processes by Fourier transform infrared spectroscopy: Helicobacter pylori drug inactivation and plasmid bioproduction in recombinant Escherichia coli culturespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.citation.conferencePlace1-4 March 2011 - Lisbon, Portugalpt_PT
oaire.citation.endPage6pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title1st Portuguese Biomedical Engineering Meetingpt_PT
oaire.fundingStream3599-PPCDT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicatione934d4a3-07d0-4c5d-8183-e76b3b0bf237
relation.isProjectOfPublication.latestForDiscoverye934d4a3-07d0-4c5d-8183-e76b3b0bf237

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