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Molecular fingerprint of human gastric cell line infected by Helicobacter pylori

2015-04-17Conference object Restricted access
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dc.contributor.authorSampaio, Pedro
dc.contributor.authorRibeiro Da Cunha, Bernardo
dc.contributor.authorRosa, Filipa
dc.contributor.authorSales, Kevin
dc.contributor.authorLopes, Marta
dc.contributor.authorCalado, Cecília
dc.date.accessioned2016-04-19T15:55:49Z
dc.date.available2016-04-19T15:55:49Z
dc.date.issued2015-04-17
dc.description.abstractHelicobacter pylori infection represents a serious health problem, given its association with serious gastric diseases as gastric ulcers, cancer and MALT lymphoma. Currently no vaccine exists and antibiotic-based eradication therapy is already failing in more than 20% of cases. To increase the knowledge on the infection process diverse gastric cell lines, e.g. the adenocarcinona gastric (AGS) cell line, are routinely used has in vitro models of gastric epithelia. In the present work the molecular fingerprint of infected and non-infected AGS cell lines, by diverse H. pylori strains, was acquired using vibrational infrared spectroscopy. These molecular fingerprints enabled to discriminate infected from non-infected AGS cells, and infection due to different strains, by performing Principal Component Analysis. It was also possible to estimate, from the AGS cells molecular fingerprint, the effect of the infection on diverse biochemical and metabolic cellular status. In resume infra-red spectroscopy enabled the acquisition of infected AGS cells molecular fingerprint with minimal sample preparation, in a rapid, high-throughput, economic process yielding highly sensitive and informative data, most useful for promoting critical knowledge on the H. pylori infection process. © 2015 IEEE.pt_PT
dc.identifier.citationSAMPAIO, Pedro N.; [et al] - Molecular fingerprint of human gastric cell line infected by Helicobacter pylori. In ENBENG 2015, IEEE 4th Portuguese Meeting on Bioengineering. Porto, Portugal: IEEE, 2015. ISBN 978-147998269-1. Pp. 1-5pt_PT
dc.identifier.doi10.1109/ENBENG.2015.7088877pt_PT
dc.identifier.isbn978-147998269-1
dc.identifier.urihttp://hdl.handle.net/10400.21/6042
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIEEE - Institute of Electrical and Electronics Engineers Inc.pt_PT
dc.relation.ispartofseries;7088877
dc.subjectAdenocarcinome gastric cell linept_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectFTIR-spectrscopypt_PT
dc.titleMolecular fingerprint of human gastric cell line infected by Helicobacter pyloript_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.endPage5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleENBENG 2015, IEEE 4th Portuguese Meeting on Bioengineeringpt_PT
person.familyNameSampaio
person.familyNameRibeiro da Cunha
person.familyNameCalado
person.givenNamePedro
person.givenNameBernardo
person.givenNameCecília
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-idEA1E-4BEA-A01E
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-0303-9416
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridP-6154-2017
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id57211629814
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication303f3e22-ec1a-4243-9ded-98c647776e6a
relation.isAuthorOfPublication810fc4c7-6c05-44a0-81e5-6cfdb3c2088a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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