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Serum albumin modulates the bioactivity of rosmarinic acid

2018-08Journal article Restricted access
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dc.contributor.authorBrito, Elsa
dc.contributor.authorSilva, André
dc.contributor.authorFale, Pedro
dc.contributor.authorPacheco, Rita
dc.contributor.authorSerralheiro, António
dc.contributor.authorHaris, Parvez I.
dc.contributor.authorAscensão, Lia
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2020-03-26T18:21:16Z
dc.date.available2020-03-26T18:21:16Z
dc.date.issued2018-08
dc.description.abstractRosmarinic acid (RA) is a phenolic compound with biological activity. The objective of the present study was to investigate whether this compound kept its biological activity in the presence of proteins. For this purpose, bovine serum albumin (BSA) was used as a model protein, and the capacity of the RA to inhibit acetylcholinesterase (AChE) and affect antioxidant activity was evaluated in the absence and presence of BSA. A mixture of phenolic compounds containing RA, obtained from a medicinal plant was added to this study. The AChE inhibitory activity of RA was reduced by *57% in the presence of BSA, while the antioxidant activity increased. These results lead to the investigation of the effect of RA on the BSA structure using Fourier transform infrared spectroscopy (FTIR). At 37 C and higher temperatures, RA caused a decrease in the temperature modifications onthe proteinstructure. Furthermore, FTIR and native-gel analysis revealed that protein aggregation/ precipitation, induced bytemperature, wasreduced in thepresence of RA. The novelty of the present work resides in thestudy of the enzyme inhibitory activity and antioxidant capacity of polyphenols, such as RA, in the presence of a protein. The findings highlight the need to consider the presence of proteins when assessing biological activities of polyphenols in vitro and that enzyme inhibitory activity may be decreased, while the antioxidant capacity remains or even increases.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBRITO, Elsa; [et al] – Serum albumin modulates the bioactivity of rosmarinic acid. Journal of Medicinal Food. ISSN 1096-620X. Vol. 21, N.º 8 (2018), pp. 801-807pt_PT
dc.identifier.doi10.1089/jmf.2017.0086pt_PT
dc.identifier.issn1096-620X.
dc.identifier.issn1557-7600
dc.identifier.urihttp://hdl.handle.net/10400.21/11350
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationUID/AMB/ 50017 - CESAMpt_PT
dc.subjectAcetylcholinesterasept_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectFTIRpt_PT
dc.subjectProtein secondary structurept_PT
dc.subjectSDS-PAGEpt_PT
dc.titleSerum albumin modulates the bioactivity of rosmarinic acidpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FQUI%2FUI0612%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.endPage807pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage801pt_PT
oaire.citation.titleJournal of Medicinal Foodpt_PT
oaire.citation.volume21pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameBrito
person.familyNameSilva
person.familyNameFale
person.familyNamePacheco
person.familyNameSerralheiro
person.familyNameAscensão
person.familyNameSerralheiro
person.givenNameElsa
person.givenNameAndré
person.givenNamePedro
person.givenNameRita
person.givenNameAntónio
person.givenNameLia
person.givenNameMaria Luisa
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-id851F-4B62-FC48
person.identifier.ciencia-id3714-4001-DCD6
person.identifier.orcid0000-0001-9552-9342
person.identifier.orcid0000-0002-5681-0614
person.identifier.orcid0000-0002-4644-7655
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-5130-909X
person.identifier.orcid0000-0001-7439-5912
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridA-5570-2013
person.identifier.ridC-3062-2012
person.identifier.ridT-5897-2017
person.identifier.ridA-2685-2014
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id25649775000
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6602262156
person.identifier.scopus-author-id6506279352
person.identifier.scopus-author-id6602688573
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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