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Nanoformulation of seaweed eisenia bicyclis in albumin nanoparticles targeting cardiovascular diseases: In vitro and in vivo evaluation

2022-09-27Journal article Open access
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dc.contributor.authorPinto, Sofia
dc.contributor.authorGaspar, Maria Manuela
dc.contributor.authorAscensão, Lia
dc.contributor.authorFaísca, Pedro
dc.contributor.authorReis, Catarina
dc.contributor.authorPacheco, Rita
dc.date.accessioned2023-05-17T13:57:18Z
dc.date.available2023-05-17T13:57:18Z
dc.date.issued2022-09-27
dc.description.abstractNatural products, especially those derived from seaweeds, are starting to be seen as effective against various diseases, such as cardiovascular diseases (CVDs). This study aimed to design a novel oral formulation of bovine albumin serum nanoparticles (BSA NPs) loaded with an extract of Eisenia bicyclis and to validate its beneficial health effects, particularly targeting hypercholesterolemia and CVD prevention. Small and well-defined BSA NPs loaded with Eisenia bicyclis extract were successfully prepared exhibiting high encapsulation efficiency. Antioxidant activity and cholesterol biosynthesis enzyme 3-hydroxy-3 methylutaryl coenzyme A reductase (HMGR) inhibition, as well as reduction of cholesterol permeation in intestinal lining model cells, were assessed for the extract both in free and nanoformulated forms. The nanoformulation was more efficient than the free extract, particularly in terms of HMGR inhibition and cholesterol permeation reduction. In vitro cytotoxicity and in vivo assays in Wistar rats were performed to evaluate its safety and overall effects on metabolism. The results demonstrated that the Eisenia bicyclis extract and BSA NPs were not cytotoxic against human intestinal Caco-2 and liver HepG2 cells and were also safe after oral administration in the rat model. In addition, an innovative approach was adopted to compare the metabolomic profile of the serum from the animals involved in the in vivo assay, which showed the extract and nanoformulation's impact on CVD-associated key metabolites. Altogether, these preliminary results revealed that the seaweed extract and the nanoformulation may constitute an alternative natural dosage form which is safe and simple to produce, capable of reducing cholesterol levels, and consequently helpful in preventing hypercholesterolemia, the main risk factor of CVDs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPINTO, Sofia; [et al] – Nanoformulation of seaweed eisenia bicyclis in albumin nanoparticles targeting cardiovascular diseases: in vitro and in vivo evaluation. Marine Drugs. eISSN 1660-3397. Vol. 20, N.º 10 (2022), pp. 1-27.pt_PT
dc.identifier.doi10.3390/md20100608pt_PT
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.21/16060
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0056/2020 - FCTpt_PT
dc.relationCentro de Química Estrutural
dc.relationCentro de Química Estrutural
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationNovel nanoplatforms for targeting melanoma with 8-hydroxyquinoline metal complexes
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/20/10/608pt_PT
dc.subjectEisenia bicyclispt_PT
dc.subjectCholesterolpt_PT
dc.subjectCardiovascular diseasespt_PT
dc.subjectNanoparticlespt_PT
dc.subjectIn vitro and in vivo evaluationpt_PT
dc.titleNanoformulation of seaweed eisenia bicyclis in albumin nanoparticles targeting cardiovascular diseases: In vitro and in vivo evaluationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleNovel nanoplatforms for targeting melanoma with 8-hydroxyquinoline metal complexes
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
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oaire.citation.endPage27pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume20pt_PT
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person.familyNamede Jesus Guilherme Gaspar
person.familyNameAscensão
person.familyNameFaisca
person.familyNameBeco Pinto Reis
person.familyNamePacheco
person.givenNameMaria Manuela
person.givenNameLia
person.givenNamePedro
person.givenNameAna Catarina
person.givenNameRita
person.identifierC-2024-2014
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person.identifier.orcid0000-0002-1046-4031
person.identifier.orcid0000-0001-5192-3006
person.identifier.ridA-2685-2014
person.identifier.ridC-3062-2012
person.identifier.scopus-author-id15834627800
person.identifier.scopus-author-id6506279352
person.identifier.scopus-author-id8853708300
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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