Repository logo
 
Publication

Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production

2010-04-28Journal article Restricted access
Simple item page
dc.contributor.authorSampaio, Pedro
dc.contributor.authorCalado, Cecília
dc.contributor.authorSOUSA, LISETE
dc.contributor.authorBressler, David C.
dc.contributor.authorPais, Maria Salomé
dc.contributor.authorFonseca, Luís P. P.
dc.date.accessioned2020-09-21T10:51:06Z
dc.date.available2020-09-21T10:51:06Z
dc.date.issued2010-04-28
dc.description.abstractThe optimization of culture medium composition was carried out for improvement the recombinant cyprosin B production, an enzyme with high milk-clotting activity. Response surface methodology (RSM) was applied to evaluate the effect of variables namely glucose, yeast extract (YE) and bactopeptone present in the culture medium, used for recombinant cyprosin B production by transformed Saccharomyces cerevisiae BJ1991 strain in shake-flask and bioreactor culture conditions. The central composite experimental design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The optimal concentration estimated for each variable related to a theoretical maximum of cyprosin B activity (488 U mL-1 ) was 30 g L-1 glucose, 15 g L-1 YE and 27 g L-1 bactopeptone. The optimized medium composition, based on empirical model, led to a cyprosin B activity of 519 U mL-1 , which corresponds to an increase of 46%. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in biomass (82%) and recombinant cyprosin B production (139%). The improvement in the recombinant cyprosin B production after optimization process can be considered adequate for large-scale applications, and the clotting activity of cyprosin B account for their use in industrial cheese making.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSAMPAIO, Pedro N.; [et al] – Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. European Food Research and Technology. ISSN 1438-2377. Vol. 231, N.º 2 (2010), pp. 339-346pt_PT
dc.identifier.doi10.1007/s00217-010-1281-zpt_PT
dc.identifier.issn1438-2377
dc.identifier.issn1438-2385
dc.identifier.urihttp://hdl.handle.net/10400.21/12247
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationSFRH/ BD/8780/2002 - FCTpt_PT
dc.relationPTDC/ MAT/64353/2006 - FCT/OEpt_PT
dc.subjectCulture media compositionpt_PT
dc.subjectCyprosin Bpt_PT
dc.subjectFermentationpt_PT
dc.subjectResponse surface methodologypt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleOptimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage346pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage339pt_PT
oaire.citation.titleEuropean Food Research and Technologypt_PT
oaire.citation.volume231pt_PT
person.familyNameSampaio
person.familyNameCalado
person.familyNameRIBEIRO DE SOUSA
person.familyNamePais
person.familyNameP. Fonseca
person.givenNamePedro
person.givenNameCecília
person.givenNameLISETE MARIA
person.givenNameMaria Salomé
person.givenNameLuis
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-id6518-7145-2F61
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2114-720X
person.identifier.orcid0000-0001-9771-2378
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication303f3e22-ec1a-4243-9ded-98c647776e6a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication95cee711-a91f-419e-83e0-d500ed0c947e
relation.isAuthorOfPublicationc335277e-1b6c-42b7-be1e-eb2557b2a8f3
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscoveryc335277e-1b6c-42b7-be1e-eb2557b2a8f3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Optimization_CRCCalado.pdf
Size:
440.1 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos