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Exploring the hypocholesterolemic potential of a fucus vesiculosus extract: Omic insights into molecular mechanisms at the intestinal level

2024-04-20Research article Open access
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authorProfile.emailbiblioteca@isel.pt
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
dc.contributor.authorAndré, Rebeca
dc.contributor.authorPacheco, Rita
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorSerralheiro, Maria Luísa
dc.date.accessioned2025-09-01T12:34:24Z
dc.date.available2025-09-01T12:34:24Z
dc.date.issued2024-04-20
dc.description.abstractHigh blood cholesterol levels are a major risk factor for cardiovascular diseases. A purified aqueous extract of Fucus vesiculosus, rich in phlorotannins and peptides, has been described for its potential to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells' metabolites and proteins was analysed to gain a deeper understanding of its mode of action on lipids' metabolism, particularly concerning the absorption and transport of exogenous cholesterol. Caco-2 cells, differentiated into enterocytes, were exposed to the extract, and analysed by untargeted metabolomics and proteomics. The results of the metabolomic analysis showed statistically significant differences in glutathione content of cells exposed to the extract compared to control cells, along with an increased expression of fatty acid amides in exposed cells. A proteomic analysis showed an increased expression in cells exposed to the extract compared to control cells of FAB1 and NPC1, proteins known to be involved in lipid metabolism and transport. To the extent of our knowledge, this study is the first use of untargeted metabolomics and a proteomic analysis to investigate the effects of F. vesiculosus on differentiated Caco-2 cells, offering insights into the molecular mechanism of the extract's compounds on intestinal cells.eng
dc.identifier.citationAndré, R., Pacheco, R., Santos, H. M., & Serralheiro, M. L. (2024). Exploring the hypocholesterolemic potential of a fucus vesiculosus extract: Omic insights into molecular mechanisms at the intestinal level. Marine Drugs, 22(4), 1-14. https://doi.org/10.3390/md22040187
dc.identifier.doihttps://doi.org/10.3390/md22040187
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.21/22077
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationBiosystems and Integrative Sciences Institute
dc.relationCentro de Química Estrutural
dc.relationUIDB/00100/2020
dc.relationLA/P/0056/2020
dc.relationPD/BD/142861/2018
dc.relationLA/P/0008/2020
dc.relationUIDP/50006/2020
dc.relationUIDB/50006/2020
dc.relation.hasversionhttps://www.mdpi.com/1660-3397/22/4/187
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFucus vesiculosus
dc.subjectProteomics
dc.subjectMetabolomics
dc.subjectNPC1
dc.subjectCaco-2 cell line
dc.titleExploring the hypocholesterolemic potential of a fucus vesiculosus extract: Omic insights into molecular mechanisms at the intestinal leveleng
dc.typeresearch article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleCentro de Química Estrutural
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.citation.endPage14
oaire.citation.issue4
oaire.citation.startPage1
oaire.citation.titleMarine Drugs
oaire.citation.volume22
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43
person.familyNamePacheco
person.givenNameRita
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0001-5192-3006
person.identifier.ridC-3062-2012
person.identifier.scopus-author-id8853708300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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