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Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells Kinetic analysis in reverse micelles

2013-09Journal article Open access
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dc.contributor.authorBernardo, Marisa
dc.contributor.authorPacheco, Rita
dc.contributor.authorSerralheiro, Maria Luísa M.
dc.contributor.authorKarmali, Amin
dc.date.accessioned2013-11-01T12:46:06Z
dc.date.available2013-11-01T12:46:06Z
dc.date.issued2013-09
dc.description.abstractIntact cells from Pseudomonas aeruginosa strain L10 containing amidase were used as biocatalysts both free and immobilized in a reverse micellar system. The apparent kinetic constants for the transamidation reaction in hydroxamic acids synthesis, were determined using substrates such as aliphatic, amino acid and aromatic amides and esters, in both media. In reverse micelles, K-m values decreased 2-7 fold relatively to the free biocatalyst using as substrates acetamide, acrylamide, propionamide and glycinamide ethyl ester. We have concluded that overall the affinity of the biocatalyst to each substrate increases when reactions are performed in the reversed micellar system as opposed to the buffer system. The immobilized biocatalyst in general, exhibits higher stability and faster rates of reactions at lower substrates concentration relatively to the free form, which is advantageous. Additionally, the immobilization revealed to be suitable for obtaining the highest yields of hydroxamic acids derivatives, in some cases higher than 80%. (C) 2013 Elsevier B.V. All rights reserved.por
dc.identifier.citationBERNARDO, Marisa; PACHECO, Rita; SERRALHEIRO, Maria Luisa M.; KARMALI, Amin - Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells Kinetic analysis in reverse micelles. Journal of Molecular Catalysis B-Enzymatic. ISSN 1381-1177. Vol. 93 (2013), p. 28-33.por
dc.identifier.issn1381-1177
dc.identifier.other10.1016/j.molcatb.2013.03.016
dc.identifier.urihttp://hdl.handle.net/10400.21/2833
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Science BVpor
dc.subjectAmidasepor
dc.subjectAcyltransferasepor
dc.subjectPseudomonas aeruginosapor
dc.subjectReverse micellespor
dc.subjectHydroxamic acidspor
dc.subjectBiocatalysispor
dc.subjectPurificationpor
dc.subjectHydrolysispor
dc.subjectInhibitorspor
dc.subjectMediapor
dc.titleProduction of hydroxamic acids by immobilized Pseudomonas aeruginosa cells Kinetic analysis in reverse micellespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceAmsterdampor
oaire.citation.endPage33por
oaire.citation.startPage28por
oaire.citation.titleJournal of Molecular Catalysis B-Enzymaticpor
oaire.citation.volume93por
person.familyNameBernardo
person.familyNamePacheco
person.familyNameKarmali
person.givenNameMarisa
person.givenNameRita
person.givenNameAmin
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0003-2661-5380
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0003-0419-401X
person.identifier.ridC-3062-2012
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id37092511100
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6603778216
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb836e2a5-3cf4-4d3d-9062-61d73a37bd64
relation.isAuthorOfPublicationd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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