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Antigenic diversity among portuguese clinical isolates of Helicobacter pylori

2011-04Journal article Restricted access
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dc.contributor.authorVitoriano, Inês
dc.contributor.authorRocha‐Gonçalves, Alexandra
dc.contributor.authorCarvalho, Teresa
dc.contributor.authorOleastro, Mónica
dc.contributor.authorCalado, Cecília
dc.contributor.authorRoxo‐Rosa, Mónica
dc.date.accessioned2020-09-21T10:18:29Z
dc.date.available2020-09-21T10:18:29Z
dc.date.issued2011-04
dc.description.abstractBackground: The human gastroduodenal pathogen, Helicobacter pylori, is characterized by an unusual extent of genetic heterogeneity. This dictates differences in the antigenic pattern of strains resulting in heterogeneous human humoral immune responses. Here, we examined the antigenic variability among a group of 10 strains isolated from Portuguese patients differing in age, gender, and H. pylori‐associated gastric diseases. Material and Methods: Immunoassays were performed on two‐dimensional electrophoresis gels obtained for the proteome of each strain, using a commercial pool of antibodies produced in rabbit, against the whole cell lysate of an Australian H. pylori strain. Relevant proteins were identified by mass spectrometry. Results: Immunoproteomes of the Portuguese strains showed no correlation between the number of antigenic proteins or the antigenic profile, and the disease to which each strain was associated. The Heat shock protein B was the unique immunoreactive protein common to all of them. Additionally, seven proteins were found to be antigenic in at least 80% of strains: enoyl‐(acyl‐carrier‐protein) reductase (NADH); Catalase; Flagellin A; 2 isoforms of alkyl hydroperoxide reductase; succinyl‐CoA transferase subunit B; and an unidentified protein. These proteins were present in the proteome of all tested strains, suggesting that differences in their antigenicity are related to antigenic variance. Conclusions: This study showed evidence of the variability of antigenic pattern among H. pylori strains. We believe that this fact contributes to the failure of anti‐H. pylori vaccines and the low accuracy of serological tests based on a low number of proteins or antigens of only one strain.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVITORIANO, Inês; [et al] – Antigenic diversity among portuguese clinical isolates of Helicobacter pylori. Helicobacter. ISSN 1523-5378. Vol. 16, N.º 2 (2011), pp. 153-168pt_PT
dc.identifier.doi10.1111/j.1523-5378.2011.00825.xpt_PT
dc.identifier.issn1523-5378
dc.identifier.urihttp://hdl.handle.net/10400.21/12246
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationEFFICIENCY IMPROVEMENT OF A DNA-BASED HELICOBACTER PYLORI VACCINE
dc.relationA BI- AND A TRI-VALENT DNA VACCINE AGAINST HELICOBACTER PYLORI
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1523-5378.2011.00825.xpt_PT
dc.subjectAntigenic diversitypt_PT
dc.subjectPortuguese strainspt_PT
dc.subjectImmunoproteomicspt_PT
dc.subject2DEpt_PT
dc.titleAntigenic diversity among portuguese clinical isolates of Helicobacter pyloript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEFFICIENCY IMPROVEMENT OF A DNA-BASED HELICOBACTER PYLORI VACCINE
oaire.awardTitleA BI- AND A TRI-VALENT DNA VACCINE AGAINST HELICOBACTER PYLORI
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F38634%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F23902%2F2005/PT
oaire.citation.endPage168pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage153pt_PT
oaire.citation.titleHelicobacterpt_PT
oaire.citation.volume16pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicatione934d4a3-07d0-4c5d-8183-e76b3b0bf237
relation.isProjectOfPublication132a7a16-73a7-4997-b565-f49feab1765f
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