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Living bacteria rheology: Population growth, aggregation patterns, and collective behaviour under diferent shear flows

dc.contributor.authorPatricio, Pedro
dc.contributor.authorAlmeida, Pedro L.
dc.contributor.authorPortela, R.
dc.contributor.authorSobral, R. G.
dc.contributor.authorGrilo, I. R.
dc.contributor.authorCidade, T.
dc.contributor.authorR. Leal, Catarina
dc.date.accessioned2015-08-25T10:30:36Z
dc.date.available2015-08-25T10:30:36Z
dc.date.issued2014-08
dc.description.abstractThe activity of growing living bacteria was investigated using real-time and in situ rheology-in stationary and oscillatory shear. Two different strains of the human pathogen Staphylococcus aureus-strain COL and its isogenic cell wall autolysis mutant, RUSAL9-were considered in this work. For low bacteria density, strain COL forms small clusters, while the mutant, presenting deficient cell separation, forms irregular larger aggregates. In the early stages of growth, when subjected to a stationary shear, the viscosity of the cultures of both strains increases with the population of cells. As the bacteria reach the exponential phase of growth, the viscosity of the cultures of the two strains follows different and rich behaviors, with no counterpart in the optical density or in the population's colony-forming units measurements. While the viscosity of strain COL culture keeps increasing during the exponential phase and returns close to its initial value for the late phase of growth, where the population stabilizes, the viscosity of the mutant strain culture decreases steeply, still in the exponential phase, remains constant for some time, and increases again, reaching a constant plateau at a maximum value for the late phase of growth. These complex viscoelastic behaviors, which were observed to be shear-stress-dependent, are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties. The viscous and elastic moduli of strain COL culture, obtained with oscillatory shear, exhibit power-law behaviors whose exponents are dependent on the bacteria growth stage. The viscous and elastic moduli of the mutant culture have complex behaviors, emerging from the different relaxation times that are associated with the large molecules of the medium and the self-organized structures of bacteria. Nevertheless, these behaviors reflect the bacteria growth stage.por
dc.identifier.citationPATRÍCIO, Pedro Manuel Alves, [et al] – Living bacteria rheology: Population growth, aggregation patterns, and collective behaviour under diferent shear flows. Physical Review E. ISSN: 1539-3755. Vol. 90, N.º. 2 (2014), pp. 1-9.por
dc.identifier.doi10.1103/PhysRevE.90.022720
dc.identifier.issn1539-3755
dc.identifier.issn1550-2376
dc.identifier.urihttp://hdl.handle.net/10400.21/4982
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmer Physical Socpor
dc.relation.ispartofseries022720
dc.subjectSoft Glassy Materialspor
dc.subjectStaphylococcus-Aureuspor
dc.subjectCellpor
dc.subjectDetachmentpor
dc.subjectResistancepor
dc.subjectInsightspor
dc.subjectBiofilmspor
dc.titleLiving bacteria rheology: Population growth, aggregation patterns, and collective behaviour under diferent shear flowspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUSApor
oaire.citation.endPage9
oaire.citation.issue2por
oaire.citation.startPage1
oaire.citation.titlePhysical Review Epor
oaire.citation.volume90por
person.familyNamePatricio
person.familyNameMarques de Almeida
person.familyNameMarques Mendes Almeida da Rosa Leal
person.givenNamePedro
person.givenNamePedro
person.givenNameCatarina
person.identifierA-2814-2009
person.identifier.ciencia-idC616-BA77-E9C9
person.identifier.ciencia-id0218-71B2-2DE0
person.identifier.ciencia-idAF15-486E-BB15
person.identifier.orcid0000-0002-9050-9956
person.identifier.orcid0000-0001-7356-8455
person.identifier.orcid0000-0001-5871-4936
person.identifier.ridA-2750-2009
person.identifier.ridB-4356-2009
person.identifier.scopus-author-id57190384213
person.identifier.scopus-author-id7005379280
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication11a2a1d5-8165-475a-8a54-e32bd8e8ce4e
relation.isAuthorOfPublication429b6d71-328e-42b3-8151-15493de249b8
relation.isAuthorOfPublication4db8990d-5eb1-4515-ae7a-68873cf776a5
relation.isAuthorOfPublication.latestForDiscovery429b6d71-328e-42b3-8151-15493de249b8

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