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Action of euptox A from Ageratina adenophora juice on human cell lines: a top-down study using FTIR spectroscopy and protein profiling

2019-06Journal article Restricted access
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dc.contributor.authorAndré, Rebeca
dc.contributor.authorCatarro, Joana
dc.contributor.authorFreitas, Dalia
dc.contributor.authorPacheco, Rita
dc.contributor.authorOliveira, Maria Conceição
dc.contributor.authorSerralheiro, Maria Luisa
dc.contributor.authorFale, Pedro
dc.date.accessioned2019-05-28T07:56:43Z
dc.date.available2019-05-28T07:56:43Z
dc.date.issued2019-06
dc.description.abstractEuptox A, from Ageratina adenophora juice, is a toxin associated with the plant's resistance to infections, invasiveness and traditional use in cancer treatment. We used FTIR spectroscopy and protein profiling of cell lines to study the impact of euptox A on human cells, to clarify its mechanism of action in a top-down approach. Euptox A was extracted from the juice of A. adenophora. Its stability in the gastrointestinal tract was evaluated, as the compound/juice is generally taken orally. Cytotoxicity was determined in HeLa, Caco-2 and MCF7 cells, and the mechanism of action analyzed by protein and metabolite profiles using electrophoresis and FTIR spectroscopy. Euptox A resisted gastrointestinal digestion and was the most cytotoxic component of the extract for all cell lines tests. Euptox A-treated HeLa cells showed changes in protein profile, especially on 40S ribosomal protein S8 (RP), generally associated with cancer cells. FTIR profiles of treated cells diverged in the same metabolites as cells treated with cisplatin, both in metabolite directed analysis and in multivariate analysis (principal component analysis). In conclusion, euptox A in this top-down study showed a cellular impact that suggests a strong potential against cancer, acting on cancer targeted cellular characteristics.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationANDRÉ, Rebeca; [et al] – Action of euptox A from Ageratina adenophora juice on human cell lines: a top-down study using FTIR spectroscopy and protein profiling. Toxicology in Vitro. ISSN 0887-2333. Vol. 57 (2019), pp. 217-225pt_PT
dc.identifier.doi10.1016/j.tiv.2019.03.012pt_PT
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/10400.21/10079
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S0887233318307872?token=F58B059AB532B9B68AB1ECC44DE50E2301289B44B102D47D459029049EAB28B2EFA6CAD7FD2AEB281C4685329486B8DFpt_PT
dc.subjectEuptox Apt_PT
dc.subjectAgeratina adenophorapt_PT
dc.subjectCancer drugpt_PT
dc.subjectFTIR spectroscopypt_PT
dc.subjectProtein profilept_PT
dc.subject40S ribosomal protein S8 (RP)pt_PT
dc.titleAction of euptox A from Ageratina adenophora juice on human cell lines: a top-down study using FTIR spectroscopy and protein profilingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage225pt_PT
oaire.citation.startPage217pt_PT
oaire.citation.titleToxicology in Vitropt_PT
oaire.citation.volume57pt_PT
person.familyNameAndré
person.familyNamePacheco
person.familyNameOliveira
person.familyNameSerralheiro
person.familyNameFale
person.givenNameRebeca
person.givenNameRita
person.givenNameMaria Conceição
person.givenNameMaria Luisa
person.givenNamePedro
person.identifier1173301
person.identifier1022927
person.identifier.ciencia-id7D18-8206-8EF7
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-idAB11-548D-A6A9
person.identifier.orcid0000-0002-2733-5797
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0002-3068-4920
person.identifier.orcid0000-0001-7541-9613
person.identifier.orcid0000-0002-4644-7655
person.identifier.ridC-3062-2012
person.identifier.ridH-8263-2012
person.identifier.ridE-3407-2012
person.identifier.ridA-5570-2013
person.identifier.scopus-author-id57207778987
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id35611565300
person.identifier.scopus-author-id6602688573
person.identifier.scopus-author-id25649775000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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