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Mid-infrared spectroscopy: a groundbreaking tool for monitoring mammalian cells processes

2017-03-30Conference object Restricted access
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dc.contributor.authorRosa, Filipa O.P.
dc.contributor.authorRibeiro Da Cunha, Bernardo
dc.contributor.authorCarmelo, Joana G.
dc.contributor.authorFernandes-Platzgummer, Ana
dc.contributor.authorda Silva, Claudia
dc.contributor.authorCalado, Cecília
dc.date.accessioned2018-01-22T09:10:30Z
dc.date.available2018-01-22T09:10:30Z
dc.date.issued2017-03-30
dc.description.abstractMammalian cells are extensively used in cell biology studies, e.g. as a model system of human pathologies, or as a major source of very high-value biopharmaceuticals (that can be the cells itself or their products e.g. heterologous proteins). As such, it is highly pertinent to develop monitoring methods for mammalian cultivations capable of delivering detailed bioprocess information in a rapid and economic way. It is relevant to acquire information concerning the conventional critical variables (as cell growth, consumption of nutrients, production and consumption of by-products and the bioproduct production), and the cell metabolism towards a better understanding of the culture process and consequently for more efficient optimization and control procedures. In the present work, Mid-infrared (MIR) spectroscopy was evaluated as a monitoring technique enabling the acquisition of said bioprocess information in a simple (single step of dehydration), rapid (minutes), economic (without reagent consumption), label-free and high-throughput mode (using 96-wells microplates). The new method was evaluated across a highly diverse set of mammalian culture processes: The monitoring of ex vivo expansion of human mesenchymal stem/stromal cells (MSC) conducted under diverse culture strategies, where it was possible to accurately predict glucose, lactate and ammonia concentrations. The monitoring of recombinant human embryonic kidney cells producing green fluorescent protein, which enabled the estimation of transfection efficiency and the metabolic impact of protein production on the host cell metabolism. Finally, the monitoring of infected gastric cell lines with Helicobacter pylori, which enabled to identify spectral biomarkers for defining the status of infection (infected vs non infected) and to characterize the infection conducted by virulent strains, usually associated to severe gastric diseases as peptide ulcer and gastric cancer. In resume, high-throughput MIR spectroscopy enabled to adequately monitor diverse mammalian cell cultures, thus allowing to attain meaningful information concerning said bioprocesses, from traditional critical variables of the process, to the metabolic status of mammalian host cells and even to define disease biomarkers in a groundbreaking way.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationROSA, Filipa O. P.; [et al] – Mid-infrared spectroscopy: A groundbreaking tool for monitoring mammalian cells processes. In 5th Portuguese Meeting on Bioengineering (ENBENG). Coimbra, Portugal: IEEE, 2017. Pp. 1-6pt_PT
dc.identifier.doi10.1109/ENBENG.2017.7889479pt_PT
dc.identifier.isbn978-1-5090-4802-1
dc.identifier.isbn978-1-5090-4801-4
dc.identifier.urihttp://hdl.handle.net/10400.21/7955
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relation.publisherversionhttp://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=7889479pt_PT
dc.subjectMonitoringpt_PT
dc.subjectPredictive modelspt_PT
dc.subjectSpectroscopypt_PT
dc.subjectProteinspt_PT
dc.subjectSugarpt_PT
dc.titleMid-infrared spectroscopy: a groundbreaking tool for monitoring mammalian cells processespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlace16-18 February - Coimbra - Portugalpt_PT
oaire.citation.endPage6pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title5th Portuguese Meeting on Bioengineering (ENBENG)pt_PT
person.familyNameRibeiro da Cunha
person.familyNameFernandes-Platzgummer
person.familyNameCalado
person.givenNameBernardo
person.givenNameAna
person.givenNameCecília
person.identifier657490
person.identifier130332
person.identifier.ciencia-idEA1E-4BEA-A01E
person.identifier.ciencia-idEA11-6777-F573
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-0303-9416
person.identifier.orcid0000-0002-5367-6528
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridP-6154-2017
person.identifier.ridF-1685-2011
person.identifier.ridK-6104-2012
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id57211629814
person.identifier.scopus-author-id35749894500
person.identifier.scopus-author-id22933449000
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication810fc4c7-6c05-44a0-81e5-6cfdb3c2088a
relation.isAuthorOfPublicatione9ec518c-41f4-4d7a-ad45-6eb2fae83693
relation.isAuthorOfPublication4ab79ed3-19e4-4b3a-85a5-7e02845201a9
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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