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Effect of Saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase

2002-11Journal article Restricted access
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dc.contributor.authorCalado, Cecília
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFonseca, Luís P. P.
dc.date.accessioned2020-10-02T10:05:08Z
dc.date.available2020-10-02T10:05:08Z
dc.date.issued2002-11
dc.description.abstractThe effect of culture media composition, and fermentation conditions and strategy on the growth and cutinase production of recombinant Saccharomyces cerevisiae and subsequent cutinase purification by expanded bed adsorption (EBA) was studied. The reduction in the amount of yeast extract used as nitrogen source from 20 g dm−3 to 10 g dm−3 in batch cultures led to a 29% decrease in the heterologous cutinase production, while the 5% cutinase dynamic adsorption capacity (q5%) on the cation Streamline SP XL was increased 6.7‐fold. By dilution of the whole fermentation broth, performed with the lowest yeast extract content, which reduces conductivity, the q5% was additionally increased by 1.9‐fold. After implementation of a fed‐batch strategy the cutinase concentration, cutinase yield on carbon source, cutinase yield on nitrogen source and productivity were increased by 10.8‐, 2.9‐, 5.3‐ and 6.4‐fold, respectively, in relation to the previously‐mentioned batch fermentation. However, the increased cutinase production was compromised by heterologous protein loss during the EBA recovery operation and the cutinase dynamic adsorption capacity and purification productivity decreased by 90% and 75%, respectively. Thus, target protein production by S cerevisiae fermentation and a downstream process with EBA cannot be considered as separate entities, where the understanding of the factors that affect the interactions among them are crucial towards optimization of the overall production process of heterologous proteins.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecília R. C.; CABRAL, Joaquim M. S.; FONSECA, Luís P. – Effect of Saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase. Journal of Chemical Technology and Biotechnology. ISSN 0268–2575. Vol. 77, N.º 11 (2002), pp. 1231-1237pt_PT
dc.identifier.doi10.1002/jctb.695pt_PT
dc.identifier.issn0268–2575
dc.identifier.urihttp://hdl.handle.net/10400.21/12271
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociety of Chemical Industrypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.695pt_PT
dc.subjectCutinasept_PT
dc.subjectExpanded bed adsorptionpt_PT
dc.subjectFermentationpt_PT
dc.subjectIntegrated processespt_PT
dc.subjectS cerevisiaept_PT
dc.titleEffect of Saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1237pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1231pt_PT
oaire.citation.titleJournal of Chemical Technology and Biotechnologypt_PT
oaire.citation.volume77pt_PT
person.familyNameCalado
person.familyNameCabral
person.familyNameP. Fonseca
person.givenNameCecília
person.givenNameJoaquim M.S.
person.givenNameLuis
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscovery5c533551-5113-4c0a-93f1-9c50cbd796bc

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