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Bioconversion of D-glucose into D-glucosone by Glucose 2-oxidase from Coriolus versicolor at Moderate Pressures

2011-04Journal article Open access
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dc.contributor.authorKarmali, Amin
dc.contributor.authorCoelho, Jose
dc.date.accessioned2013-01-30T18:50:34Z
dc.date.available2013-01-30T18:50:34Z
dc.date.issued2011-04
dc.description.abstractGlucose 2-oxidase (pyranose oxidase, pyranose: oxygen-2-oxidoreductase, EC 1.1.3.10) from Coriolus versicolor catalyses the oxidation of D-glucose at carbon 2 in the presence of molecular O(2) producing D-glucosone (2-keto-glucose and D-arabino-2-hexosulose) and H(2)O(2). It was used to convert D-glucose into D-glucosone at moderate pressures (i.e. up to 150 bar) with compressed air in a modified commercial batch reactor. Several parameters affecting biocatalysis at moderate pressures were investigated as follows: pressure, [enzyme], [glucose], pH, temperature, nature of fluid and the presence of catalase. Glucose 2-oxidase was purified by immobilized metal affinity chromatography on epoxy-activated Sepharose 6B-IDA-Cu(II) column at pH 6.0. The rate of bioconversion of D-glucose increased with the pressure since an increase in the pressure with compressed air resulted in higher rates of conversion. On the other hand, the presence of catalase increased the rate of reaction which strongly suggests that H(2)O(2) acted as inhibitor for this reaction. The rate of bioconversion of D-glucose by glucose 2-oxidase in the presence of either nitrogen or supercritical CO(2) at 110 bar was very low compared with the use of compressed air at the same pressure. The optimum temperature (55 degrees C) and pH (5.0) of D-glucose bioconversion as well as kinetic parameters for this enzyme were determined under moderate pressure. The activation energy (E(a)) was 32.08 kJmol(-1) and kinetic parameters (V(max), K(m), K(cat) and K(cat)/K(m)) for this bioconversion were 8.8 Umg(-1) protein, 2.95 mM, 30.81 s(-1) and 10,444.06 s(-1)M(-1), respectively. The biomass of C. versicolor as well as the cell-free extract containing glucose 2-oxidase activity were also useful for bioconversion of D-glucose at moderate pressures. The enzyme was apparently stable at moderate pressures since such pressures did not affect significantly the enzyme activity.por
dc.identifier.citationKARMALI, Amin; COELHO, José - Bioconversion of D-glucose into D-glucosone by Glucose 2-oxidase from Coriolus versicolor at ModeratePressures. Applied Biochemistry and Biotechnology. ISSN 0273-2289. Vol. 163, n.º 7 (2011) p. 906-917.por
dc.identifier.issn0273-2289
dc.identifier.urihttp://hdl.handle.net/10400.21/2109
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherHumana Press Incpor
dc.subjectBiocatalysispor
dc.subjectModerate pressurespor
dc.subjectGlucose 2-oxidasepor
dc.subjectCoriolus versicolorpor
dc.subjectBioconversion of D-Glucose into D-glucosonepor
dc.subjectCatalasepor
dc.subjectKinetic parameterspor
dc.subjectPyranose 2-oxidasepor
dc.subjectTrametes-multicolorpor
dc.subjectPeniophora-giganteapor
dc.subjectMolecular-cloningpor
dc.subjectFungal strainspor
dc.subjectPurificationpor
dc.subjectOxidasepor
dc.subjectBasidiomycetepor
dc.subjectBehaviorpor
dc.titleBioconversion of D-glucose into D-glucosone by Glucose 2-oxidase from Coriolus versicolor at Moderate Pressurespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceTotowapor
oaire.citation.endPage917por
oaire.citation.issue7por
oaire.citation.startPage906por
oaire.citation.titleApplied Biochemistry and Biotechnologypor
oaire.citation.volume163por
person.familyNameKarmali
person.familyNameCoelho
person.givenNameAmin
person.givenNameJose
person.identifierR-000-84M
person.identifier.ciencia-idD716-31DF-8371
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0001-8118-0864
person.identifier.ridE-4787-2011
person.identifier.ridG-2626-2011
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id7101841005
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationec13b922-f442-44fa-bb07-7c2e0bebbe6d
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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