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Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy

2019-03-05Journal article Restricted access
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dc.contributor.authorMarques, Vanda
dc.contributor.authorRibeiro Da Cunha, Bernardo
dc.contributor.authorCouto, Andreia
dc.contributor.authorSampaio, Pedro
dc.contributor.authorFonseca, Luís P. P.
dc.contributor.authorAleixo, Sandra
dc.contributor.authorCalado, Cecília
dc.date.accessioned2018-11-23T09:55:23Z
dc.date.available2018-11-23T09:55:23Z
dc.date.issued2019-03-05
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL
dc.description.abstractThe infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMARQUES, Vanda; [et al] – Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN: 1386-1425. Vol 210, (2019), pp. 193-202pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.saa.2018.11.001pt_PT
dc.identifier.issn1386-1425
dc.identifier.urihttp://hdl.handle.net/10400.21/9079
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2017/DrugsPlatf/ISELpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S1386142518309922?token=41457B7BE2E8422F4C09F7892D3AC3EACACC59EFB1970D3C9996272875BDED5020C4BB12369D40D9592C77315A50A44Apt_PT
dc.subjectInfrared spectroscopypt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectGastric adenocarcinoma cellspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectEspectroscopia de infravermelhopt_PT
dc.subjectCélulas de adenocarcinoma gástricopt_PT
dc.subjectBiomarcadorespt_PT
dc.titleCharacterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAT%2F00006%2F2013/PT
oaire.citation.endPage202pt_PT
oaire.citation.startPage193pt_PT
oaire.citation.titleSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopypt_PT
oaire.citation.volume210pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameMarques
person.familyNameRibeiro da Cunha
person.familyNameSampaio
person.familyNameP. Fonseca
person.familyNameAleixo
person.familyNameCalado
person.givenNameVanda
person.givenNameBernardo
person.givenNamePedro
person.givenNameLuis
person.givenNameSandra
person.givenNameCecília
person.identifier904979
person.identifier1251979
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person.identifier.ciencia-id951D-DF38-3397
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-3515-0781
person.identifier.orcid0000-0002-0303-9416
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0001-8429-6977
person.identifier.orcid0000-0003-1740-8371
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridB-9607-2019
person.identifier.ridP-6154-2017
person.identifier.ridA-4228-2013
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id37059182200
person.identifier.scopus-author-id57211629814
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id55916288400
person.identifier.scopus-author-id24829443600
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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