Publication
Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy
dc.contributor.author | Marques, Vanda | |
dc.contributor.author | Ribeiro Da Cunha, Bernardo | |
dc.contributor.author | Couto, Andreia | |
dc.contributor.author | Sampaio, Pedro | |
dc.contributor.author | Fonseca, Luís P. P. | |
dc.contributor.author | Aleixo, Sandra | |
dc.contributor.author | Calado, Cecília | |
dc.date.accessioned | 2018-11-23T09:55:23Z | |
dc.date.available | 2018-11-23T09:55:23Z | |
dc.date.issued | 2019-03-05 | |
dc.description | Este trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2017 do Instituto Politécnico de Lisboa. Código de referência IPL/2017/DrugsPlatf/ISEL | |
dc.description.abstract | The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | MARQUES, Vanda; [et al] – Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN: 1386-1425. Vol 210, (2019), pp. 193-202 | pt_PT |
dc.identifier.doi | https://doi.org/10.1016/j.saa.2018.11.001 | pt_PT |
dc.identifier.issn | 1386-1425 | |
dc.identifier.uri | http://hdl.handle.net/10400.21/9079 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | Projeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2017/DrugsPlatf/ISEL | pt_PT |
dc.relation.publisherversion | https://reader.elsevier.com/reader/sd/pii/S1386142518309922?token=41457B7BE2E8422F4C09F7892D3AC3EACACC59EFB1970D3C9996272875BDED5020C4BB12369D40D9592C77315A50A44A | pt_PT |
dc.subject | Infrared spectroscopy | pt_PT |
dc.subject | Helicobacter pylori | pt_PT |
dc.subject | Gastric adenocarcinoma cells | pt_PT |
dc.subject | Biomarkers | pt_PT |
dc.subject | Espectroscopia de infravermelho | pt_PT |
dc.subject | Células de adenocarcinoma gástrico | pt_PT |
dc.subject | Biomarcadores | pt_PT |
dc.title | Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMAT%2F00006%2F2013/PT | |
oaire.citation.endPage | 202 | pt_PT |
oaire.citation.startPage | 193 | pt_PT |
oaire.citation.title | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | pt_PT |
oaire.citation.volume | 210 | pt_PT |
oaire.fundingStream | 3599-PPCDT | |
oaire.fundingStream | 5876 | |
person.familyName | Marques | |
person.familyName | Ribeiro da Cunha | |
person.familyName | Sampaio | |
person.familyName | P. Fonseca | |
person.familyName | Aleixo | |
person.familyName | Calado | |
person.givenName | Vanda | |
person.givenName | Bernardo | |
person.givenName | Pedro | |
person.givenName | Luis | |
person.givenName | Sandra | |
person.givenName | Cecília | |
person.identifier | 904979 | |
person.identifier | 1251979 | |
person.identifier | 130332 | |
person.identifier.ciencia-id | 4716-E0F9-1EC7 | |
person.identifier.ciencia-id | EA1E-4BEA-A01E | |
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person.identifier.ciencia-id | 951D-DF38-3397 | |
person.identifier.ciencia-id | 9418-E320-3177 | |
person.identifier.orcid | 0000-0003-3515-0781 | |
person.identifier.orcid | 0000-0002-0303-9416 | |
person.identifier.orcid | 0000-0003-2917-4904 | |
person.identifier.orcid | 0000-0001-8429-6977 | |
person.identifier.orcid | 0000-0003-1740-8371 | |
person.identifier.orcid | 0000-0002-5264-9755 | |
person.identifier.rid | B-9607-2019 | |
person.identifier.rid | P-6154-2017 | |
person.identifier.rid | A-4228-2013 | |
person.identifier.rid | E-2102-2014 | |
person.identifier.scopus-author-id | 37059182200 | |
person.identifier.scopus-author-id | 57211629814 | |
person.identifier.scopus-author-id | 24178064100 | |
person.identifier.scopus-author-id | 55916288400 | |
person.identifier.scopus-author-id | 24829443600 | |
person.identifier.scopus-author-id | 6603163260 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | closedAccess | pt_PT |
rcaap.type | article | pt_PT |
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