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Comparative analysis of near and mid-infrared spectroscopy to monitor recombinant cyprosin production

2015-04-20Conference object Restricted access
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dc.contributor.authorSampaio, Pedro
dc.contributor.authorCalado, Cecília
dc.date.accessioned2016-04-21T10:25:43Z
dc.date.available2016-04-21T10:25:43Z
dc.date.issued2015-04-20
dc.description.abstractInfrared spectroscopy, either in the near and mid (NIR/MIR) region of the spectra, has gained great acceptance in the industry for bioprocess monitoring according to Process Analytical Technology, due to its rapid, economic, high sensitivity mode of application and versatility. Due to the relevance of cyprosin (mostly for dairy industry), and as NIR and MIR spectroscopy presents specific characteristics that ultimately may complement each other, in the present work these techniques were compared to monitor and characterize by in situ and by at-line high-throughput analysis, respectively, recombinant cyprosin production by Saccharomyces cerevisiae. Partial least-square regression models, relating NIR and MIR-spectral features with biomass, cyprosin activity, specific activity, glucose, galactose, ethanol and acetate concentration were developed, all presenting, in general, high regression coefficients and low prediction errors. In the case of biomass and glucose slight better models were achieved by in situ NIR spectroscopic analysis, while for cyprosin activity and specific activity slight better models were achieved by at-line MIR spectroscopic analysis. Therefore both techniques enabled to monitor the highly dynamic cyprosin production bioprocess, promoting by this way more efficient platforms for the bioprocess optimization and control.pt_PT
dc.identifier.citationSAMPAIO, Pedro N.; CALADO, Cecília, C. R. - Comparative analysis of near and mid-infrared spectroscopy to monitor recombinant cyprosin production. In ENBENG 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineering. Porto, Portugal: IEEE, 2015. ISBN: 978-1-4799-8269-1. Pp. 1-5pt_PT
dc.identifier.doi10.1109/ENBENG.2015.7088876pt_PT
dc.identifier.isbn978-1-4799-8269-1
dc.identifier.urihttp://hdl.handle.net/10400.21/6070
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIEEE - Institute of Electrical and Electronics Engineers Inc.pt_PT
dc.subjectMid infrared spectroscopypt_PT
dc.subjectNear infrared spectroscopypt_PT
dc.subjectRecombinant cyprosinpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleComparative analysis of near and mid-infrared spectroscopy to monitor recombinant cyprosin productionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.endPage5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleENBENG 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineeringpt_PT
person.familyNameSampaio
person.familyNameCalado
person.givenNamePedro
person.givenNameCecília
person.identifier1251979
person.identifier130332
person.identifier.ciencia-idAF12-6ABA-43D8
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0003-2917-4904
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id24178064100
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication303f3e22-ec1a-4243-9ded-98c647776e6a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscovery303f3e22-ec1a-4243-9ded-98c647776e6a

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