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A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase

2023-11-25Research article Restricted access
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authorProfile.emailbiblioteca@isel.pt
datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
dc.contributor.authorSilva, Diogo
dc.contributor.authorSousa, Ana Catarina
dc.contributor.authorRobalo, Maria Paula
dc.contributor.authorMartins, Lígia O.
dc.date.accessioned2025-09-01T11:40:02Z
dc.date.available2025-09-01T11:40:02Z
dc.date.issued2023-11-25
dc.description.abstractLignin is the second most abundant natural polymer next to cellulose and by far the largest renewable source of aromatic compounds on the planet. Dye-decolourising peroxidases (DyPs) are biocatalysts with immense potential in lignocellulose biorefineries to valorize emerging lignin building blocks for environmentally friendly chemicals and materials. This work investigates the catalytic potential of the engineered PpDyP variant 6E10 for the oxidation of 24 syringyl, guaiacyl and hydroxybenzene lignin-phenolic derivatives. Variant 6E10 exhibited up to 100-fold higher oxidation rates at pH 8 for all the tested phenolic substrates compared to the wild-type enzyme and other acidic DyPs described in the literature. The main products of reactions were dimeric isomers with molecular weights of (2 × MWsubstrate - 2 H). Their structure depends on the substitution pattern of the aromatic ring of substrates, i.e., of the coupling possibilities of the primarily formed radicals upon enzymatic oxidation. Among the dimers identified were syringaresinol, divanillin and diapocynin, important sources of structural scaffolds exploitable in medicinal chemistry, food additives and polymers.eng
dc.identifier.citationSilva, D., Sousa, A. C., Robalo, M. P., & Martins, L. O. (2023). A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase. New Biotechnology, 77, 176-184. https://doi.org/10.1016/j.nbt.2022.12.003
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2022.12.003
dc.identifier.eissn1876-4347
dc.identifier.issn1876-4347
dc.identifier.urihttp://hdl.handle.net/10400.21/22076
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationPTDC/BBBEBB/0122/2014
dc.relationPTDC/BII-BBF/ 29564/2017
dc.relationUIDB/04612/2020
dc.relationUIDP/04612/2020
dc.relationLA/P/0087/2020
dc.relationUIDB/00100/2020
dc.relationUIDP/00100/2020
dc.relationLA/P/0056/2020
dc.relationSFRH/BD/132702/2017
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S187167842200067X?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOxidoreductases
dc.subjectBiocatalysis
dc.subjectBiorefineries
dc.subjectLignin valorization
dc.subjectLignans
dc.subjectNatural products
dc.titleA wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidaseeng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage184
oaire.citation.startPage176
oaire.citation.titleNew Biotechnology
oaire.citation.volume77
oaire.versionhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43
person.familyNameSousa
person.familyNameRobalo
person.givenNameAna Catarina
person.givenNameMaria Paula
person.identifier.ciencia-idE81E-0596-BF29
person.identifier.ciencia-idE11C-A704-9C18
person.identifier.orcid0000-0001-6133-7406
person.identifier.orcid0000-0002-8200-6910
person.identifier.ridU-3597-2017
person.identifier.ridB-2002-2012
person.identifier.scopus-author-id9269662100
relation.isAuthorOfPublication2ec1bb85-33cd-4e24-b84f-0c965d84d25b
relation.isAuthorOfPublication823a9498-17ab-451f-bb8f-56338643ac0c
relation.isAuthorOfPublication.latestForDiscovery2ec1bb85-33cd-4e24-b84f-0c965d84d25b

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