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Adsorption of myoglobin on calixarenes and biocatalysis in organic media

2011Journal article Restricted access
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dc.contributor.authorSemedo, Magda C.
dc.contributor.authorKarmali, Amin
dc.contributor.authorBarata, Patrícia
dc.contributor.authorPrata, José V.
dc.date.accessioned2020-06-08T17:46:13Z
dc.date.available2020-06-08T17:46:13Z
dc.date.issued2011
dc.description.abstractDerivatives of p-tert-butylcalix[4,6,8]arene carboxylic acids were used for selective adsorption of myoglobin.Amixtureofmyoglobin,laccaseandperoxidase wasusedforextractionwithcalixarenesandonlymyoglobin was selectively extracted to organic media. Myoglobin and Mb c–calixarene exhibited pseudoactivity of peroxidase in aqueous and organic media. This protein-calixarene complex exhibited the highest specific activity of 1.37 × 10−1 U.mg protein−1 at initial pH 6.5 of myoglobin aqueous solution. Apparent kinetic parameters (V max, K m, k cat and k cat/K m) for the pseudoperoxidase activity were determined in organic media for different initial pH values of myoglobin aqueous solution by Michaelis-Menten plot. The stability of this complex was studied for different initial pH values and t1/2 values were obtained in the range of 3.5–5.2 days. The extracted Mb c in organic media was recovered into fresh aqueous solutions at alkaline pH with a recovery of pseudoperoxidase activity of over 100%.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSEMEDO, Magda C.; [et al] – Adsorption of myoglobin on calixarenes and biocatalysis in organic media. Journal of Advanced Chemical Engineering. ISSN 2090-4568. Vol. 1 (2011), pp. 1-10pt_PT
dc.identifier.doi10.4303/jace/A110401pt_PT
dc.identifier.issn2090-4568
dc.identifier.urihttp://hdl.handle.net/10400.21/11796
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAshdin Publishingpt_PT
dc.subjectCalixarenespt_PT
dc.subjectProtein adsorptionpt_PT
dc.subjectMyoglobinpt_PT
dc.subjectBiocatalysis in organic mediapt_PT
dc.subjectPseudoperoxidase activitypt_PT
dc.titleAdsorption of myoglobin on calixarenes and biocatalysis in organic mediapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Advanced Chemical Engineeringpt_PT
oaire.citation.volume1pt_PT
person.familyNameSemedo
person.familyNameKarmali
person.familyNameBarata
person.familyNamePrata
person.givenNameMagda
person.givenNameAmin
person.givenNamePatrícia
person.givenNameJosé Virgílio
person.identifier1022083
person.identifier1090399
person.identifier191765
person.identifier.ciencia-id9C11-BD02-C77F
person.identifier.ciencia-idC019-9F30-0F99
person.identifier.ciencia-idC11A-9130-8981
person.identifier.orcid0000-0001-6330-2899
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0001-8274-633X
person.identifier.orcid0000-0002-9068-2847
person.identifier.ridE-4787-2011
person.identifier.ridB-7214-2008
person.identifier.scopus-author-id57000381900
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id8968446000
person.identifier.scopus-author-id9276778400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication15e229f3-a619-4931-87eb-799091dc1601
relation.isAuthorOfPublication.latestForDiscovery23975b4b-3234-4139-9675-89ea6f9a2332

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