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Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract: Omic Insights into Molecular Mechanisms at the Intestinal Level

2024-04-20Journal article Open access
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dc.contributor.authorRebeca, André
dc.contributor.authorPacheco, Rita
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2024-11-11T10:56:29Z
dc.date.available2024-11-11T10:56:29Z
dc.date.issued2024-04-20
dc.description.abstractHigh blood cholesterol levels are a major risk factor for cardiovascular diseases. A purified aqueous extract of Fucus vesiculosus, rich in phlorotannins and peptides, has been described for its potential to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells' metabolites and proteins was analysed to gain a deeper understanding of its mode of action on lipids' metabolism, particularly concerning the absorption and transport of exogenous cholesterol. Caco-2 cells, differentiated into enterocytes, were exposed to the extract, and analysed by untargeted metabolomics and proteomics. The results of the metabolomic analysis showed statistically significant differences in glutathione content of cells exposed to the extract compared to control cells, along with an increased expression of fatty acid amides in exposed cells. A proteomic analysis showed an increased expression in cells exposed to the extract compared to control cells of FAB1 and NPC1, proteins known to be involved in lipid metabolism and transport. To the extent of our knowledge, this study is the first use of untargeted metabolomics and a proteomic analysis to investigate the effects of F. vesiculosus on differentiated Caco-2 cells, offering insights into the molecular mechanism of the extract’s compounds on intestinal cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAndré R, Pacheco R, Santos HM, Serralheiro ML. Exploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract: Omic Insights into Molecular Mechanisms at the Intestinal Level. Marine Drugs. 2024; 22(4):187. https://doi.org/10.3390/md22040187pt_PT
dc.identifier.doi10.3390/md22040187pt_PT
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.21/17868
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationLA/P/0056/2020 - Institute of Molecular Sciences is an Associate Laboratory funded by FCTpt_PT
dc.relationBiosystems and Integrative Sciences Institute
dc.relationBiosystems and Integrative Sciences Institute
dc.relationCentro de Química Estrutural
dc.relationCentro de Química Estrutural
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/22/4/187pt_PT
dc.subjectFucus vesiculosuspt_PT
dc.subjectproteomicspt_PT
dc.subjectmetabolomicspt_PT
dc.subjectNPC1pt_PT
dc.subjectCaco-2 cell linept_PT
dc.titleExploring the Hypocholesterolemic Potential of a Fucus vesiculosus Extract: Omic Insights into Molecular Mechanisms at the Intestinal Levelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00100%2F2020/PT
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oaire.citation.endPage14pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
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person.familyNamePacheco
person.familyNameSerralheiro
person.givenNameRita
person.givenNameMaria Luisa
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6602688573
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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