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Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production

2011-11Journal article Restricted access
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dc.contributor.authorSampaio, P. N.
dc.contributor.authorSOUSA, LISETE
dc.contributor.authorCalado, Cecília
dc.contributor.authorPais, M. S.
dc.contributor.authorFonseca\, L. P.
dc.date.accessioned2020-09-18T10:20:30Z
dc.date.available2020-09-18T10:20:30Z
dc.date.issued2011-11
dc.description.abstractTwo multivariate statistical methods, factor analysis (FA) and hierarchical cluster analysis (HCA), were applied to experimental data set to evaluate their usefulness in selecting the adequate expression system and optimal growth parameters for recombinant cyprosin B production. Using FA, the large data set was reduced to two factors representing 73.4% of variability. Factor 1, with 53.5% of variability, corresponds to recombinant cyprosin B expression and efficient secretion, while factor 2, accounting for 19.9% of variability, represents cell growth and physiological characteristics. FA and HCA allowed the establishment of correlations among different variables and the clusters obtained providing clear identification of the experimental parameters related to cyprosin B production, which results on more accurate scientific output and time saving when selection of an adequate expression system is concerned.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSAMPAIO, P. N.; [et al] – Use of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B production. Biotechnology Letters. ISSN 0141-5492. Vol. 33, N.º 11 (2011), pp. 2111-2119pt_PT
dc.identifier.doi10.1007/s10529-011-0678-5pt_PT
dc.identifier.issn1573-6776
dc.identifier.issn0141-5492
dc.identifier.urihttp://hdl.handle.net/10400.21/12245
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationPROCESSOS INTEGRADOS DE PRODUÇÃO E RECUPERAÇÃO IN SITU DE CIPROZINAS POR LEVEDURAS RECOMBINANTES
dc.relation.publisherversionhttps://link.springer.com/content/pdf/10.1007/s10529-011-0678-5.pdfpt_PT
dc.subjectChemometricspt_PT
dc.subjectCyprosin Bpt_PT
dc.subjectFactor analysispt_PT
dc.subjectHierarchical cluster analysispt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.titleUse of chemometrics in the selection of a Saccharomyces cerevisiae expression system for recombinant cyprosin B productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePROCESSOS INTEGRADOS DE PRODUÇÃO E RECUPERAÇÃO IN SITU DE CIPROZINAS POR LEVEDURAS RECOMBINANTES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F8780%2F2002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAT%2F64353%2F2006/PT
oaire.citation.endPage2119pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage2111pt_PT
oaire.citation.titleBiotechnology Letterspt_PT
oaire.citation.volume33pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameRIBEIRO DE SOUSA
person.familyNameCalado
person.givenNameLISETE MARIA
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id6518-7145-2F61
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-2114-720X
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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