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Development of a high-throughput monitoring technique of bacteria photodynamic inactivation

2015-04-20Conference object Restricted access
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dc.contributor.authorRibeiro Da Cunha, Bernardo
dc.contributor.authorSampaio, Pedro N.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2016-04-19T15:28:06Z
dc.date.available2016-04-19T15:28:06Z
dc.date.issued2015-04-20
dc.description.abstractSummary form only given. Bacterial infections and the fight against them have been one of the major concerns of mankind since the dawn of time. During the `golden years' of antibiotic discovery, during the 1940-90s, it was thought that the war against infectious diseases had been won. However currently, due to the drug resistance increase, associated with the inefficiency of discovering new antibiotic classes, infectious diseases are again a major public health concern. A potential alternative to antibiotic treatments may be the antimicrobial photodynamic inactivation (PDI) therapy. To date no indication of antimicrobial PDI resistance development has been reported. However the PDI protocol depends on the bacteria species [1], and in some cases on the bacteria strains, for instance Staphylococcus aureus [2]. Therefore the development of PDI monitoring techniques for diverse bacteria strains is critical in pursuing further understanding of such promising alternative therapy. The present works aims to evaluate Fourier-Transformed-Infra-Red (FT-IR) spectroscopy to monitor the PDI of two model bacteria, a gram-negative (Escherichia coli) and a gram-positive (S. aureus) bacteria. For that a high-throughput FTIR spectroscopic method was implemented as generally described in Scholz et al. [3], using short incubation periods and microliter quantities of the incubation mixture containing the bacteria and the PDI-drug model the known bactericidal tetracationic porphyrin 5,10,15,20-tetrakis (4-N, N, Ntrimethylammoniumphenyl)-porphyrin p-tosylate (TTAP4+). In both bacteria models it was possible to detect, by FTIR-spectroscopy, the drugs effect on the cellular composition either directly on the spectra or on score plots of principal component analysis. Furthermore the technique enabled to infer the effect of PDI on the major cellular biomolecules and metabolic status, for example the turn-over metabolism. In summary bacteria PDI was monitored in an economic, rapid (in minutes- , high-throughput (using microplates with 96 wells) and highly sensitive mode resourcing to FTIR spectroscopy, which could serve has a technological basis for the evaluation of antimicrobial PDI therapies efficiency.pt_PT
dc.identifier.citationCUNHA, Bernardo R.; [et al] - Development of a high-throughput monitoring technique of bacteria photodynamic inactivation. In ENBENG 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineering. Porto, Portugal: IEEE, 2015. ISBN 978-1-4799-8269-1. Pp. 1-2pt_PT
dc.identifier.doi10.1109/ENBENG.2015.7088827pt_PT
dc.identifier.isbn978-1-4799-8269-1
dc.identifier.urihttp://hdl.handle.net/10400.21/6041
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIEEE - Institute of Electrical and Electronics Engineers Inc.pt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectInfra-red spectroscopypt_PT
dc.subjectPhotodynamic therapypt_PT
dc.titleDevelopment of a high-throughput monitoring technique of bacteria photodynamic inactivationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.endPage2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleENBENG 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineeringpt_PT
person.familyNameRibeiro da Cunha
person.familyNameCalado
person.givenNameBernardo
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-idEA1E-4BEA-A01E
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-0303-9416
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridP-6154-2017
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id57211629814
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication810fc4c7-6c05-44a0-81e5-6cfdb3c2088a
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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