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Finding value in wastewaters from the cork industry: carbon dots synthesis and fluorescence for hemeprotein detection

2020-05-02Journal article Open access
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dc.contributor.authorAlexandre, Marta R.
dc.contributor.authorCosta, Alexandra I.
dc.contributor.authorBerberan-Santos, Mario
dc.contributor.authorPrata, José V.
dc.date.accessioned2020-06-05T14:06:26Z
dc.date.available2020-06-05T14:06:26Z
dc.date.issued2020-05-02
dc.description.abstractValorisation of industrial low-value waste residues was preconized. Hence, carbon dots (C-dots) were synthesized from wastewaters of the cork industry—an abundant and a_ordable, but environmentally-problematic industrial e_uent. The carbon nanomaterials were structurally and morphologically characterised, and their photophysical properties were analysed by an ensemble of spectroscopy techniques. Afterwards, they were successfully applied as highly-sensitive fluorescence probes for the direct detection of haemproteins. Haemoglobin, cytochrome c and myoglobin were selected as specific targets owing to their relevant roles in living organisms, wherein their deficiencies or surpluses are associated with several medical conditions. For all of them, remarkable responses were achieved, allowing their detection at nanomolar levels. Steady-state and time-resolved fluorescence, ground-state UV–Vis absorption and electronic circular dichroism techniques were used to investigate the probable mechanisms behind the fluorescence turn-o_ of C-dots. Extensive experimental evidence points to a static quenching mechanism. Likewise, resonance energy transfer and collisional quenching have been discarded as excited-state deactivating mechanisms. It was additionally found that na oxidative, photoinduced electron transfer occurs for cytochrome c, the most electron-deficient protein Besides, C-dots prepared from citric acid/ethylenediamine were comparatively assayed for protein detection and the di_erences between the two types of nanomaterials highlighted.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationALEXANDRE, Marta R.; [et al] – Finding value in wastewaters from the cork industry: carbon dots synthesis and fluorescence for hemeprotein detection. Molecules. ISSN 1420-3049. Vol. 25, N.º 10 (2020), pp. 1-18pt_PT
dc.identifier.doi10.3390/molecules25102320pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.21/11781
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUIDB/00616/2020 - FCT/MCTESpt_PT
dc.relationUIDP/00616/2020 - FCT/MCTESpt_PT
dc.subjectCorkpt_PT
dc.subjectWastewaterpt_PT
dc.subjectCarbon dotspt_PT
dc.subjectFluorescencept_PT
dc.subjectSensorpt_PT
dc.subjectHaemoglobinpt_PT
dc.subjectMyoglobinpt_PT
dc.subjectCytochrome cpt_PT
dc.titleFinding value in wastewaters from the cork industry: carbon dots synthesis and fluorescence for hemeprotein detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage18pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume25pt_PT
person.familyNameCosta
person.familyNameBerberan-Santos
person.familyNamePrata
person.givenNameAlexandra
person.givenNameMario
person.givenNameJosé Virgílio
person.identifier191765
person.identifier.ciencia-id4212-F7BF-D971
person.identifier.ciencia-id3010-007D-E2FC
person.identifier.ciencia-idC11A-9130-8981
person.identifier.orcid0000-0002-5518-6506
person.identifier.orcid0000-0002-2946-1498
person.identifier.orcid0000-0002-9068-2847
person.identifier.ridB-3810-2008
person.identifier.ridB-7214-2008
person.identifier.scopus-author-id8968446100
person.identifier.scopus-author-id7004298586
person.identifier.scopus-author-id9276778400
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5f3891cc-260d-41d3-b803-4fd17e3734bd
relation.isAuthorOfPublicationb5bf327c-3da9-456d-a0bd-b316a282e527
relation.isAuthorOfPublication15e229f3-a619-4931-87eb-799091dc1601
relation.isAuthorOfPublication.latestForDiscoveryb5bf327c-3da9-456d-a0bd-b316a282e527

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