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Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high- throughput Fourier transform infrared spectroscopy

2017-10Journal article Restricted access
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dc.contributor.authorSousa, Pedro N.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2017-11-10T10:21:03Z
dc.date.available2017-11-10T10:21:03Z
dc.date.issued2017-10
dc.description.abstractThis study shows the application of the Fourier transform mid-infrared spectroscopy (FT-MIR) associated with high-throughput technology to study the biochemical fingerprints of different Saccharomyces cerevisiae strains transformed with the same expression system along the similar cultivation in bioreactor. The phenotype, as well as the cellular metabolism and recombinant cyprosin biosynthesis, were determined. The differences observed were confirmed by conventional cyprosin activity protocol, and the metabolic evolution was analyzed using high-performance liquid chromatography technique. The spectral analysis based on chemometrics tools, such as the principal component analysis, is a useful methodology for the phenotypes characterization as well as the specific metabolic states along the cultivations according to the clusters created. The ratio bands of spectra also represented a useful tool to evaluate the metabolic and biochemical differences between both expression systems, allowing to have an additional parameter to the biomolecular comparison. Therefore, high-throughput FT-MIR spectroscopy associated with multivariate data analysis represent a valuable strategy for extracting significant specific biomolecular information along the cultivation, providing a complete bioprocess analysis, once it detects slight molecular changes which it will be useful for screening and optimization process in the biotechnological or pharmaceutical industry.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSAMPAIO, Pedro N. Sousa; CALADO, Cecília Ribeiro da Cruz - Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high- throughput Fourier transform infrared spectroscopy. Journal of Biotechnology. ISSN 0168-1656. Vol. 260 (2017), pp. 1-10pt_PT
dc.identifier.doi10.1016/j.jbiotec.2017.08.020pt_PT
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.urihttp://hdl.handle.net/10400.21/7477
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier Sciencept_PT
dc.relationPTDC/QEQERQ/1648/2014,pt_PT
dc.relationUID/QUI/00100/2013.pt_PT
dc.relation.publisherversionhttps://ac.els-cdn.com/S0168165617316243/1-s2.0-S0168165617316243-main.pdf?_tid=fd1302cc-c56c-11e7-abf3-00000aab0f6c&acdnat=1510245935_54f7eac48148e4dcd9a079a58d0414fept_PT
dc.subjectFT-MIR spectroscopypt_PT
dc.subjectHigh-throughput analysispt_PT
dc.subjectPrincipal components analysispt_PT
dc.subjectRecombinant cyprosin Bpt_PT
dc.subjectScreening processpt_PT
dc.titleComparative analysis of different transformed Saccharomyces cerevisiae strains based on high- throughput Fourier transform infrared spectroscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQEQ-QIN%2F3967%2F2014/PT
oaire.citation.endPage10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.volume260pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicationeaa9460b-be21-49e6-b4bb-c330e07621f9
relation.isProjectOfPublication.latestForDiscoveryeaa9460b-be21-49e6-b4bb-c330e07621f9

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