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Rheology of living cells

2019-04-18Conference object Open access
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dc.contributor.authorPortela, R.
dc.contributor.authorFranco, Jose M.
dc.contributor.authorAlmeida, Pedro L.
dc.contributor.authorPatricio, Pedro
dc.contributor.authorSobral, Rita
dc.contributor.authorR. Leal, Catarina
dc.date.accessioned2020-04-15T17:08:39Z
dc.date.available2020-04-15T17:08:39Z
dc.date.issued2019-04-18
dc.description.abstractThe mechanical behavior of living cells, during planktonic growth, has been thoroughly explored combining common biological techniques with rheology and rheo-imaging measurements. Under a shear flow, bacterial cultures of Staphylococcus aureus revealed a complex and rich rheological behavior not usually accessed in biological studies. In particular, in stationary shear flow, the viscosity increased during the exponential phase and returned close to its initial value at the late phase of growth, accompanied by the stabilization of the bacterial population. In oscillatory flow, the elastic and viscous moduli exhibited power-law behaviors whose exponents are dependent on the bacteria growth stage, and can be associated to a Soft Glassy Material behavior. These behaviors were framed in a microscopic model that suggests the formation of a dynamic web-like structure, where specific aggregation phenomena may occur, depending on growth stage and cell density. Furthermore, systematic measurements combining optical density and dry weight techniques presented new evidences, which confirmed that the observed cell aggregation patterns developed during growth, under shear, can not only be cell density dependent.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPORTELA, R. – Rheology of living cells. In 6th IEEE Portuguese Meeting on Bioengineering (ENBENG 2019). Lisbon, Portugal: IEEE, 2019. ISBN 978-1-5386-8507-5. Pp. 1-4pt_PT
dc.identifier.isbn978-1-5386-8507-5
dc.identifier.isbn978-1-5386-8506-8
dc.identifier.urihttp://hdl.handle.net/10400.21/11455
dc.language.isoengpt_PT
dc.publisherIEEE EMBS Portugal Chapterpt_PT
dc.relationPTDC/FIS- NAN/0117/2014 - FCTpt_PT
dc.relationPOCI-01-0145- FEDER-007728 - ERDF under the PT2020 Partnership Agreementpt_PT
dc.subjectRheologypt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectBiological studiespt_PT
dc.subjectOscillatory flowpt_PT
dc.subjectSoft Glassy Materialpt_PT
dc.subjectSpecific aggregation phenomenapt_PT
dc.subjectGrowth stagept_PT
dc.subjectCell densitypt_PT
dc.titleRheology of living cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCTM%2F50025%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.citation.conferencePlace22-23 February 2019 - Lisbon,Portugalpt_PT
oaire.citation.endPage4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title6th IEEE Portuguese Meeting on Bioengineering (ENBENG 2019)pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameFranco
person.familyNameMarques de Almeida
person.familyNamePatricio
person.familyNameSobral
person.familyNameMarques Mendes Almeida da Rosa Leal
person.givenNameJose M.
person.givenNamePedro
person.givenNamePedro
person.givenNameRita
person.givenNameCatarina
person.identifierA-2814-2009
person.identifier.ciencia-id0218-71B2-2DE0
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person.identifier.ciencia-id7F1F-7412-A2C3
person.identifier.ciencia-idAF15-486E-BB15
person.identifier.orcid0000-0002-3165-394X
person.identifier.orcid0000-0001-7356-8455
person.identifier.orcid0000-0002-9050-9956
person.identifier.orcid0000-0003-4533-7531
person.identifier.orcid0000-0001-5871-4936
person.identifier.ridK-2809-2014
person.identifier.ridB-4356-2009
person.identifier.ridA-2750-2009
person.identifier.ridH-4710-2011
person.identifier.scopus-author-id57200084076
person.identifier.scopus-author-id57190384213
person.identifier.scopus-author-id6507285825
person.identifier.scopus-author-id7005379280
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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