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Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system

2011-09-23Conference object Open access
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dc.contributor.authorGonçalves, Lídia M. D.
dc.contributor.authorCadete, Ana
dc.contributor.authorFigueiredo, Lara
dc.contributor.authorCalado, Cecília
dc.contributor.authorAlmeida, António J.
dc.date.accessioned2020-07-31T12:44:30Z
dc.date.available2020-07-31T12:44:30Z
dc.date.issued2011-09-23
dc.description.abstractThe delivery of nucleic acids via the oral route involves overcoming barriers such as degradation of nucleic acids by low pH in the stomach, enzymatic degradation by DNases in the gut, crossing the physical barrier imposed by the mucus layer, cellular uptake, intracellular trafficking and nuclear uptake. As an oral drug carrier system chitosan nanoparticles are ideal, being mucoadhesive, interacting with the anionic sialic acid residues in mucin. In this study, plasmid DNA expressing a humanized secreted Gaussia Luciferase as reporter gene was encapsulated in alginate and chitosan nanoparticles, via a mild ionotropic gelation procedure with sodium tripolyphosphate as a counterion. The nanoparticle system here developed shows effective transfection of different human gastric epithelial cell lines with distinct cell differention. That was confirmed by the expression of luciferase in the different tested conditions, particularly the amount of encapsulated pGLuc.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGONÇALVES, Lídia M. D.; [et al] – Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system. In 1st Portuguese Biomedical Engineering Meeting. Lisbon, Portugal: IEEE, 2011. ISBN 978-1-4577-0522-9. Pp. 1-4pt_PT
dc.identifier.doi10.1109/ENBENG.2011.6026051pt_PT
dc.identifier.isbn978-1-4577-0522-9
dc.identifier.isbn978-1-4577-0523-6
dc.identifier.isbn978-1-4577-0521-2
dc.identifier.urihttp://hdl.handle.net/10400.21/12130
dc.language.isoengpt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6026051pt_PT
dc.subjectPlasmidpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectChitosanpt_PT
dc.subjectCellpt_PT
dc.subjectTranfectionpt_PT
dc.titleBiodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery systempt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.citation.conferencePlace1-4 March 2011 - Lisbon, Portugalpt_PT
oaire.citation.endPage4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title1st Portuguese Biomedical Engineering Meetingpt_PT
oaire.fundingStream3599-PPCDT
person.familyNameCadete
person.familyNameCalado
person.givenNameAna
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-3853-3895
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscovery3bdbe94c-edc1-44fe-9db0-152c5b41d01d
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relation.isProjectOfPublication.latestForDiscoverye934d4a3-07d0-4c5d-8183-e76b3b0bf237

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