Repository logo
 
Publication

Melanin: production from cheese bacteria, chemical characterization, and biological activities

2021-10-09Journal article Open access
Simple item page
dc.contributor.authorFerraz, Ana Rita
dc.contributor.authorPacheco, Rita
dc.contributor.authorVaz, Pedro D.
dc.contributor.authorPintado, C. M. B. S.
dc.contributor.authorAscensão, Lia
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2021-11-18T14:40:00Z
dc.date.available2021-11-18T14:40:00Z
dc.date.issued2021-10-09
dc.description.abstractPigments are compounds of importance to several industries, for instance, the food industry, where they can be used as additives, color intensifiers, and antioxidants. As the current trend around the world is shifting to the use of eco-friendly commodities, demand for natural dyes is increasing. Melanins are pigments that are produced by several microorganisms. Pseudomonas putida ESACB 191, isolated from goat cheese rind, was described as a brown pigment producer. This strain produces a brown pigment via the synthetic Mueller-Hinton Broth. This brown compound was extracted, purified, analyzed by FTIR and mass spectrometry, and identified as eumelanin. The maximum productivity was 1.57 mg/L/h. The bioactivity of eumelanin was evaluated as the capacity for scavenging free radicals (antioxidant activity), EC50 74.0 & PLUSMN; 0.2 mu g/mL, and as an acetylcholinesterase inhibitor, with IC50 575 & PLUSMN; 4 mu g/mL. This bacterial eumelanin did not show cytotoxicity towards A375, HeLa Kyoto, HepG2, or Caco2 cell lines. The effect of melanin on cholesterol absorption and drug interaction was evaluated in order to understand the interaction of melanin present in the cheese rind when ingested by consumers. However, it had no effect either on cholesterol absorption through an intestinal simulated barrier formed by the Caco2 cell line or with the drug ezetimibe.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFERRAZ, Ana Rita; [et al] – Melanin: production from cheese bacteria, chemical characterization, and biological activities. International Journal of Environmental Research and Public Health. eISSN 1660-4601. Vol. 18, N.º 20 (2021), pp. 1-18pt_PT
dc.identifier.doi10.3390/ijerph182010562pt_PT
dc.identifier.eissn1660-4601
dc.identifier.urihttp://hdl.handle.net/10400.21/14014
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationUID/AMB/00681/2019 - FCT with financial support to IPCB-CERNASpt_PT
dc.relationUIDB/04046/2020 - BioISI Research Unitpt_PT
dc.relationUIDP/50017/2020 - CESAMpt_PT
dc.relationUIDB/50017/2020 - CESAMpt_PT
dc.relationPTDC/Bia-BQM/28355/2017 - FCTpt_PT
dc.relationSFRH/BD/135692/2018 - FCTpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1660-4601/18/20/10562pt_PT
dc.subjectBacterial productspt_PT
dc.subjectPseudomonas spp.pt_PT
dc.subjectEumelaninpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectAcetylcholinesterase inhibitionpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectCholesterolpt_PT
dc.titleMelanin: production from cheese bacteria, chemical characterization, and biological activitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage18pt_PT
oaire.citation.issue20pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleInternational Journal of Environmental Research and Public Healthpt_PT
oaire.citation.volume18pt_PT
person.familyNameFerraz
person.familyNamePacheco
person.familyNameVaz
person.familyNamePintado
person.familyNameAscensão
person.familyNameSerralheiro
person.givenNameAna Rita
person.givenNameRita
person.givenNamePedro D.
person.givenNameCristina
person.givenNameLia
person.givenNameMaria Luisa
person.identifier1744600
person.identifier1022927
person.identifier.ciencia-id0919-6473-8399
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-id9B12-4313-E8F8
person.identifier.ciencia-id7F1F-E7E1-5BC6
person.identifier.ciencia-id3714-4001-DCD6
person.identifier.orcid0000-0002-8356-907X
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-9720-9801
person.identifier.orcid0000-0001-5433-4258
person.identifier.orcid0000-0001-7439-5912
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridD-7317-2012
person.identifier.ridA-2685-2014
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id36726548100
person.identifier.scopus-author-id6602633775
person.identifier.scopus-author-id6506279352
person.identifier.scopus-author-id6602688573
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0ff86486-1153-47ae-a6c7-0ff06bfc8004
relation.isAuthorOfPublicationd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isAuthorOfPublication8aa5fada-bbd1-4dbf-b53b-51bbc0eca722
relation.isAuthorOfPublication38f7ae16-5a8a-4db4-ba8c-444e6148c5af
relation.isAuthorOfPublication846ad192-df18-4b83-928d-4a4b1d6ac664
relation.isAuthorOfPublication88895442-2a46-4878-a83f-435152a9cbb4
relation.isAuthorOfPublication.latestForDiscovery8aa5fada-bbd1-4dbf-b53b-51bbc0eca722

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Melanin_RPacheco.pdf
Size:
5.94 MB
Format:
Adobe Portable Document Format
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos