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Novel insights into the biological activity of Croton lechleri twigs extracts and advancements in their sustainable recovery

2024-09-02Journal article Open access
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dc.contributor.authorTzintzarov, Alexander
dc.contributor.authorBoyadzhieva, Stanislava S.
dc.contributor.authorCoelho, Jose
dc.contributor.authorTsvetanova, Flora
dc.contributor.authorPetrova, Maria
dc.contributor.authorStoev, Georgi
dc.contributor.authorYankov, Dragomir
dc.contributor.authorUgrinova, Iva
dc.contributor.authorStateva, Roumiana
dc.date.accessioned2024-11-12T11:33:52Z
dc.date.available2024-11-12T11:33:52Z
dc.date.issued2024-09-02
dc.description.abstractSangre de drago, the sap of Croton lechleri Müll. Arg. tree, has been used for centuries in traditional medicine owing to its diverse biological activities. Extracts derived from different parts of the species contain a multitude of phytochemicals with varied applications. Twigs, however, are among the least studied parts of the plant. This study unveils new biological activities of Croton lechleri twig extracts recovered by applying Soxhlet and advanced green techniques. For all extracts, total phenolic content and antioxidant activity were determined. Subsequently, four were selected, and their cytotoxic effects were assessed on both normal (HaCat) and malignant melanoma (A375) skin cell lines using the MTT assay and trypan blue exclusion assay. All showed dose-dependent cytotoxicity, with the Soxhlet ethanol extract demonstrating the highest selectivity towards A375 cells over HaCat cells. The extracts induced apoptosis and necrosis, as confirmed by Annexin V/PI dual-labeling and flow cytometry, highlighting their ability to trigger programmed cell death in cancer cells. The selective inhibition of cell cycle progression in A375 compared to HaCat observed both for Soxhlet ethanol and pressurized ethanol extracts induces cell cycle arrest at multiple points, primarily in the G1 and G2/M phases, and significantly reduces DNA synthesis as evidenced by the decrease in the S-phase population, confirmed by the EdU assay. Consequently, the Soxhlet extract composition was analyzed using LC-MS, which revealed their richness in polyphenolic compounds, particularly flavonoids from the flavonol subclass.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTzintzarov A, Boyadzhieva SS, Coelho JAP, Tsvetanova F, Petrova M, Stoev G, Yankov DS, Ugrinova I, Stateva RP. Novel Insights into the Biological Activity of Croton lechleri Twigs Extracts and Advancements in Their Sustainable Recovery. Molecules. 2024; 29(17):4161. https://doi.org/10.3390/molecules29174161pt_PT
dc.identifier.doi10.3390/molecules29174161pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.21/17887
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/29/17/4161pt_PT
dc.subjectCroton lechleri twig extractspt_PT
dc.subjectsupercriticalpt_PT
dc.subjectpressurized liquid extractionspt_PT
dc.subjectcytotoxicitypt_PT
dc.subjectselectivity indexpt_PT
dc.subjecttotal phenolic and flavonoid contentpt_PT
dc.subjectantioxidant activitypt_PT
dc.titleNovel insights into the biological activity of Croton lechleri twigs extracts and advancements in their sustainable recoverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage30pt_PT
oaire.citation.issue17pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume29pt_PT
person.familyNameCoelho
person.familyNameTsvetanova
person.familyNamePetrova
person.familyNameYankov
person.familyNameUgrinova
person.familyNameStateva
person.givenNameJose
person.givenNameFlora
person.givenNameMaria
person.givenNameDragomir
person.givenNameIva
person.givenNameRoumiana
person.identifierR-000-84M
person.identifier1407566
person.identifier624772
person.identifier.ciencia-idD716-31DF-8371
person.identifier.orcid0000-0001-8118-0864
person.identifier.orcid0000-0003-3101-8388
person.identifier.orcid0000-0003-0766-3934
person.identifier.orcid0000-0003-0433-7930
person.identifier.orcid0000-0002-0116-0079
person.identifier.orcid0000-0002-4834-8183
person.identifier.ridG-2626-2011
person.identifier.ridG-8711-2011
person.identifier.scopus-author-id7101841005
person.identifier.scopus-author-id57213649255
person.identifier.scopus-author-id56548632500
person.identifier.scopus-author-id6507848814
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationf29f8912-6df5-4f63-959d-b3998a9a8c28
relation.isAuthorOfPublication.latestForDiscovery9599ed34-bb6e-4828-97d3-b1ad76cc7f14

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