Repository logo
 
Publication

A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase

2022-12Journal article Open access
Simple item page
dc.contributor.authorSilva, Diogo
dc.contributor.authorSousa, Ana Catarina
dc.contributor.authorRobalo, M.P.
dc.contributor.authorMartins, Lígia O.
dc.date.accessioned2023-02-03T10:43:33Z
dc.date.available2023-02-03T10:43:33Z
dc.date.issued2022-12
dc.description.abstractLignin is the second most abundant natural polymer next to cellulose and by far the largest renewable source of aromatic compounds on the planet. Dye-decolourising peroxidases (DyPs) are biocatalysts with immense potential in lignocellulose biorefineries to valorize emerging lignin building blocks for environmentally friendly chemicals and materials. This work investigates the catalytic potential of the engineered PpDyP variant 6E10 for the oxidation of 24 syringyl, guaiacyl and hydroxybenzene lignin-phenolic derivatives. Variant 6E10 exhibited up to 100-fold higher oxidation rates at pH 8 for all the tested phenolic substrates compared to the wild-type enzyme and other acidic DyPs described in the literature. The main products of reactions were dimeric isomers with molecular weights of (2 × MWsubstrate - 2 H). Their structure depends on the substitution pattern of the aromatic ring of substrates, i.e., of the coupling possibilities of the primarily formed radicals upon enzymatic oxidation. Among the dimers identified were syringaresinol, divanillin and diapocynin, important sources of structural scaffolds exploitable in medicinal chemistry, food additives and polymers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSILVA, Diogo; SOUSA, A. C.; ROBALO, M. Paula; MARTINS, L.O. - A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase. New Biotechnology. ISSN 1871-6784. (2022) p. 1-9.pt_PT
dc.identifier.doi10.1016/j.nbt.2022.12.003pt_PT
dc.identifier.issn1871-6784
dc.identifier.urihttp://hdl.handle.net/10400.21/15475
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLA/P/0056/2020)pt_PT
dc.relationCentro de Química Estrutural
dc.relationLA/P/0087/2020)pt_PT
dc.relationTool-box of engineered DyP-type peroxidases for lignin degradation and valorisation
dc.relationMOSTMICRO-ITQBpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S187167842200067X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOxidoreductasespt_PT
dc.subjectBiocatalysispt_PT
dc.subjectBiorefineriespt_PT
dc.subjectLignin valorizationpt_PT
dc.subjectLignanspt_PT
dc.subjectNatural productspt_PT
dc.titleA wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleTool-box of engineered DyP-type peroxidases for lignin degradation and valorisation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F132702%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBII-BBF%2F29564%2F2017/PT
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleNew Biotechnologypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStream9471 - RIDTI
person.familyNameRobalo
person.givenNameMaria Paula
person.identifier.ciencia-idE11C-A704-9C18
person.identifier.orcid0000-0002-8200-6910
person.identifier.ridB-2002-2012
person.identifier.scopus-author-id9269662100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication823a9498-17ab-451f-bb8f-56338643ac0c
relation.isAuthorOfPublication.latestForDiscovery823a9498-17ab-451f-bb8f-56338643ac0c
relation.isProjectOfPublication4ca2f791-5aae-48f8-ac70-bde2e34d442a
relation.isProjectOfPublication597e6da3-cd8e-4b24-9bb6-c9233e7dfa76
relation.isProjectOfPublication5c587751-4559-4256-99ec-b25761cbf2a5
relation.isProjectOfPublication.latestForDiscovery597e6da3-cd8e-4b24-9bb6-c9233e7dfa76

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase.pdf
Size:
1.78 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos