Browsing by Author "Vasconcelos, Jocelyne Neto"
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- A implementação de um rastreio neonatal de doença das células falciformes em Angola: apoio à formação de técnicos de saúde no âmbito da cooperação Portugal-AngolaPublication . Brito, Miguel; Ginete, Catarina; Inusa, Baba; Mendes, Manuela; Vasconcelos, Jocelyne Neto; Veiga, LuisaIntrodução: A doença das células falciformes (DCF) é uma doença hereditária severa, com uma incidência especialmente elevada na África subsariana, sendo responsável por 50% da mortalidade em crianças com menos de 5 anos. A prevalência desta doença genética em Angola ronda os 2%, sendo a doença genética mais frequentes no mundo. A identificação da doença à nascença permite o seguimento das crianças em consultas especializadas reduzindo assim a mortalidade e morbilidade infantil. Objetivos: Implementação de um Rastreio neonatal em Luanda para diagnóstico de DCF, com treino na recolha e análise de amostras bem como apoio no follow up dos pacientes e redução da taxa de mortalidade abaixo dos 5 anos. Pretende-se ainda avaliar os custos da implementação do rastreio neonatal, bem como avaliar o impacto financeiro do diagnóstico precoce. Contexto: O nosso objetivo é a implementação do Rastreio Neonatal no Hospital Materno Infantil Dr. Manuel Pedro Azancot de Menezes, Luanda. Pretendemos realizar Treino in loco dos profissionais de saúde (técnicos de laboratório, médicos, enfermeiros e auxiliares de saúde) no processo de recolha de amostras e de dados, bem como, na deslocação de técnicos de Laboratório a Lisboa para formação na técnica de electroforese de hemoglobinas por focagem isoeléctrica no equipamento Migele (adquirido com o apoio da Perkin Elmer e do projeto ARISE). Os estágios em Lisboa estão integrados no projeto “ARISE- African Research and Innovative Initiative for Sickle cell Education”, financiado pela União Europeia no programa Marie Skłodowska-Curie Actions. Pretende-se que no futuro todo o processo relativo ao rastreio neonatal seja assegurado em Luanda pelos técnicos formados no nosso laboratório.
- Implementation of a newborn screening for sickle cell disease, at the Hospital Materno Infantil Dr Manuel Pedro Azancot de Menezes, AngolaPublication . Brito, Miguel; Inusa, Baba P.; Ginete, Catarina; Mendes, Manuela; Afonso, Roberto; Valentim, Isabel; Siatembo, Adriano; Vasconcelos, Jocelyne NetoSickle Cell Disease (SCD) is a hereditary disease, and a worldwide public health problem, with a higher incidence in sub-Saharan Africa, where the prevalence is very high (about 80%). SCD prevalence can reach 3,3 % in Angola, while carriers of the sickle cell trait account for more than 25% of the population. The early identification of patients and their follow-up in consultation are an added value for the reduction of morbidity and mortality of children with SCD. The implementation of early infant screening and follow-up programs for children with SCD showed excellent results in increasing the survival of these children. With this project we intend to implement a Newborn screening in Hospital Materno Infantil Dr Manuel Pedro Azancot de Menezes, a referral of the main maternity in Angola, located in Luanda, the country capital, that could be replicated in other hospitals, giving training to the health professionals, and to the laboratory technicians, to diagnose the SCD patients. The objective of this project is to establish a neonatal screening for SCD at a main Hospital in Angola, contributing to reducing under-5 mortality and supporting pediatric follow-up consultations of children diagnosed with SCD. Specific aims include: recording the data and medical history of babies diagnosed with SCD up to 5 years of age at regular appointments; estimating the prevalence of SCD in the municipality of Camama, Luanda; and evaluating the costs of neonatal screening and early interventions in Angola, by supporting a registry of SCD.
- Sickle cell disease and gut health: the influence of intestinal parasites and the microbiome on Angolan childrenPublication . Delgadinho, Mariana; Ginete, Catarina; Santos, Brígida; Vasconcelos, Jocelyne Neto; Arez, Ana Paula; Brito, MiguelParasitic infections are a common problem in developing countries and can intensify morbidity in patients with sickle cell disease (SCD), increasing the severity of anemia and the need for transfusions. It has been demonstrated that both helminths and protozoa can affect gut microbiome composition. On the other hand, the presence of specific bacterial communities can also influence parasite establishment. Considering this, our aim was to associate the presence of intestinal parasites with the results of hematological analyses and microbiome composition evaluations in a population of Angolan children with and without SCD. A total of 113 stool samples were collected, and gut microbiome analysis was performed using 16S sequencing and real-time PCR to detect eight different intestinal parasites. In our population, more than half of children (55%) had at least one parasitic infection, and of these, 43% were co-infected. Giardia intestinalis and Ascaris lumbricoides were more frequently found in children from the rural area of Bengo. Moreover, SCD children with ascariasis exhibited higher values of leukocytes and neutrophils, whereas the total hemoglobin levels were lower. In regards to the gut microbiome, the presence of intestinal parasites lowered the prevalence of some beneficial bacteria, namely: Lactobacillus, Bifidobacterium, Cuneatibacter, Bacteroides uniformis, Roseburia, and Shuttleworthia. This study presents the prevalence of several intestinal parasites in a high-risk transmission area with scarce information and opens new perspectives for understanding the interaction between parasites, the microbiome, and SCD.