Browsing by Author "Tavares, A."
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- Assessment the activity incorporated by the patient’s caregivers after Iodine-131 thyroid therapyPublication . Raminhas Carapinha, Maria João; Reis, M.; Silva, L.; Santos, M.; Costa, G.; Silva, M.; Isidoro, J.; Nunes, Carla; Tavares, A.; Teles, P.Aim of the study - Evaluate the activity incorporated by the caregivers of the patients undergoing thyroid cancer (TC) therapy with iodine-131 (131I), based on the amount of 131I excreted in the urine.
- Effect of technical parameters on dose and image quality in a computed radiography systemPublication . Tavares, A.; Lança, Luís; Machado, NunoThe discovery of X-rays was undoubtedly one of the greatest stimulus for improving the efficiency in the provision of healthcare services. The ability to view, non-invasively, inside the human body has greatly facilitated the work of professionals in diagnosis of diseases. The exclusive focus on image quality (IQ), without understanding how they are obtained, affect negatively the efficiency in diagnostic radiology. The equilibrium between the benefits and the risks are often forgotten. It is necessary to adopt optimization strategies to maximize the benefits (image quality) and minimize risk (dose to the patient) in radiological facilities. In radiology, the implementation of optimization strategies involves an understanding of images acquisition process. When a radiographer adopts a certain value of a parameter (tube potential [kVp], tube current-exposure time product [mAs] or additional filtration), it is essential to know its meaning and impact of their variation in dose and image quality. Without this, any optimization strategy will be a failure. Worldwide, data show that use of x-rays has been increasingly frequent. In Cabo Verde, we note an effort by healthcare institutions (e.g. Ministry of Health) in equipping radiological facilities and the recent installation of a telemedicine system requires purchase of new radiological equipment. In addition, the transition from screen-films to digital systems is characterized by a raise in patient exposure. Given that this transition is slower in less developed countries, as is the case of Cabo Verde, the need to adopt optimization strategies becomes increasingly necessary. This study was conducted as an attempt to answer that need. Although this work is about objective evaluation of image quality, and in medical practice the evaluation is usually subjective (visual evaluation of images by radiographer / radiologist), studies reported a correlation between these two types of evaluation (objective and subjective) [5-7] which accredits for conducting such studies. The purpose of this study is to evaluate the effect of exposure parameters (kVp and mAs) when using additional Cooper (Cu) filtration in dose and image quality in a Computed Radiography system.
- Estudo do interactoma da proteína MOB1 de Toxoplasma gondii usando ferramentas bioinformáticas e tecnologia BioIDPublication . Delgado, I. L.; Tavares, A.; Francisco, S.; Santos, D.; Zúquete, S.; Leitão, A.; Soares, Helena; Nolasco, SofiaO protozoário Toxoplasma gondii é um parasita intracelular obrigatório pertencente ao filo Apicomplexa, único pela sua capacidade de infetar qualquer vertebrado. As cinases MOB integram o módulo nuclear da Hippo (humano) e da mitotic exit network (levedura), vias sinalizadoras reguladoras de citocinese, proliferação celular e apoptose, em eucariotas. Através de BLAST, identificámos uma proteína MOB1 em T. gondii. Combinando as bases de dados STRING e ToxoDB.org e a tecnologia BLAST, identificámos como prováveis interactores da proteína MOB1 em T. gondii: 6 proteínas homólogas de membros nucleares das vias Hippo/MEN (YAP, TAZ, LATS/Dbf2, MST/Cdc15, Tem1, Nud1) e 7 proteínas relacionadas com sinalização celular (cinases e ativadores de GTPase). Esta rede de proteínas constitui uma base de trabalho para a caracterização dos mecanismos reguladores do ciclo celular de T. gondii. Para identificar experimentalmente interactores da MOB1, utilizámos a técnica BioID. Consiste na expressão da proteína de interesse em fusão com BirA que irá biotinilar proteínas na sua proximidade. Construímos duas estirpes transgénicas de T. gondii a sobrexpressar as proteínas de fusão Myc-BirA (controlo) e Myc-MOB1-BirA (teste). Isolámos as proteínas biotiniladas por imunoprecipitação com streptavidina e a sua identificação foi feita por espectrometria de massa. Detetámos 3 proteínas ainda não caracterizadas em T. gondii: 2 proteínas transmembranares e uma proteína com domínios funcionais relacionados com a manutenção estrutural de cromossomas. Apesar destas proteínas não terem sido identificadas pelos métodos bioinformáticos, já foram descritas, em outros organismos, proteínas MOB no centrossoma/corpo polar do fuso, cinetocóros e fuso acromático. Os resultados bioinformáticos indicam que as vias sinalizadoras Hippo/mitotic exit network poderão ser ativas em T. gondii, apoiando o provável papel de MOB1 na replicação de T. gondii. Porém, os resultados experimentais, embora preliminares, sugerem que neste parasita estas proteínas poderão ter papéis ainda não descritos em outros organismos. Estão em curso experiências adicionais para testar estes candidatos a interactores da MOB1.
- Personal dose equivalent HP(10) in patient’s family members after 131I therapy in thyroid cancer and benign thyroid diseasesPublication . Raminhas Carapinha, Maria João; Ferreira, T. C.; Rézio, M. T.; Salgado, L.; Vaz, S.; Costa, G.; Ferreira, R.; Isidoro, J.; Nunes, Carla; Tavares, A.Aim of the study - The aim is to quantify the personal equivalent dose Hp(10) of the family members (FMs) of patients submitted to iodine-131 therapy (RAIT): thyroid cancer (TC) or benign thyroid diseases (BTD) and compare Hp(10) values of children and adults.