Percorrer por autor "Santos, Joana"
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- Efeitos do treino orientado para a tarefa na marcha, equilíbrio e medo de cair após acidente vascular cerebral: estudo de casoPublication . Refacho, André; Santos, Joana; Magalhães, Maria; Lourenço, Teresa; Fernandes, BeatrizIntrodução – O acidente vascular cerebral (AVC) está intimamente relacionado com o aumento da ocorrência de quedas, assim como o medo de cair, também este um fator predisponente para quedas. As alterações ao nível do equilíbrio e da marcha, presentes até em fases mais crónicas, provocam o aumento do risco de quedas nesta população. Objetivo – Investigar os efeitos do treino orientado para a tarefa na marcha, equilíbrio e medo de cair num paciente com AVC em fase crónica. Métodos – O presente estudo seguiu uma metodologia de estudo de caso, tendo sido realizada uma avaliação inicial, seguida de uma intervenção durante sete semanas, baseada em treino orientado para a tarefa. Os testes utilizados foram a Escala Internacional de Eficácia de Quedas (FES-I), a Escala de Equilíbrio de Berg (EEB), o Índice de Barthel (IB), o Timed Up and Go Test (TUG) e o Teste de Velocidade de Marcha de quatro metros (TVM4). Resultados – Observaram-se melhorias clínicas na FES-1, EEB, Índice de Barthel, TUG e TVM4. Discussão/Conclusão – Em 14 sessões de programa baseado no treino orientado para a tarefa observaram-se ganhos com importância clínica a nível do equilíbrio, marcha e medo de cair num indivíduo em fase crónica após AVC. As melhorias no equilíbrio e funcionalidade da marcha foram acompanhadas do aumento da confiança na realização das atividades da vida diária.
- Effect of culturing human embryos in group on the composition of spent culture mediaPublication . Santos, Joana; Ramalhete, Luís; Gallardo, Miguel; Calado, CecíliaIn assisted reproductive technologies the number of human embryos maintained together per culture drop will affect the embryo development and embryo fitness for implantation and live birth. In the present work, it was evaluated how the number of embryos per culture drop affects the molecular fingerprint of spent culture media used till blastocyst stage (achieved at day 5 or 6), and consequently indirectly the embryo metabolism. It was considered embryos cultured in sequential media, with media changing at day 3, and maintained individually (n=19) or in groups of 2 (n=6) or 3 (n=21) embryos per culture drop. It was observed by principal component analysis of the second derivative infrared spectra that media at day 3 presented a different fingerprint than media at day 5 or 6, independently of the number of embryos maintained together. This highlight the differences between the two media and the high metabolic changes that occurs with the embryo metabolism till the blastocyst development stage. It was also identified specific ratios between spectral bands, either at day 3 either at day 5/6, that discriminate at a significance of 10%, cultures of individual embryos from embryos maintained in groups, and embryos maintained in groups of 3 in relation to embryos maintained in groups of 2. These results highlight the high impact of the number of embryos maintained in the same culture drop, in the culture media composition and consequently on the embryo metabolism. It was possible to identify molecular signatures specific to the culture time and number of embryos per culture drop, pointing to the high sensitivity and specificity of infrared spectroscopy to capture indirectly the embryo metabolism by non-invasive analysis of the spent culture media.
- One health approach to tackle microbial contamination on poultries: a systematic reviewPublication . Gomes, Bianca; Dias, Marta; Cervantes, Renata; Pena, Pedro; Santos, Joana; Vasconcelos Pinto, Marta; Viegas, CarlaThis study reports the search of available data published regarding microbial occupational exposure assessment in poultries, following the PRISMA methodology. Air collection through filtration was the most frequently used. The most commonly used passive sampling method was material collection such as dust, cages, soils, sediment, and wastewater. Regarding assays applied, the majority of studies comprised culture-based methods, but molecular tools were also frequently used. Screening for antimicrobial susceptibility was performed only for bacteria; cytotoxicity, virological and serological assays were also performed. Most of the selected studies focused on bacteria, although fungi, endotoxins, and β-glucans were also assessed. The only study concerning fungi and mycotoxins reported the carcinogenic mycotoxin AFB1. This study gives a comprehensive overview of microbial contamination in the poultry industry, emphasizing this setting as a potential reservoir of microbial pathogens threatening human, animal, and environmental health. Additionally, this research helps to provide a sampling and analysis protocol proposal to evaluate the microbiological contamination in these facilities. Few articles were found reporting fungal contamination in poultry farms worldwide. In addition, information concerning fungal resistance profile and mycotoxin contamination remains scarce. Overall, a One Health approach should be incorporated into exposure assessments and the knowledge gaps identified in this paper should be addressed in further research.
- The introduction of a group embryo culture protocol in donor oocyte-recipient cycles did not increase the total useable blastocyst rates: a retrospective studyPublication . Barradas-Ribeiro, Inês; Santos, Joana; Braula-Reis, Ana; Castro, Miriam; Fernandes, Micaela; Ferreira, Pedro; Metello, José Luís; Soares, Ana Paula; Calado, Cecília; Santos-Ribeiro, Samuel; Gallardo, MiguelGroup culture of human preimplantation embryos could increase the number of useable blastocysts per in vitro fertilization (IVF) cycle. The present study compares the outcomes of a specific group embryo culture strategy vs. single culture of 1 embryo per media droplet.