Logo do repositório

Percorrer por autor "Loeffler, Juergen"

A mostrar 1 - 2 de 2
  • Antifungal-resistant mucorales in different indoor environments
    Publication . Aranha Caetano, Liliana; Faria, Tiago; Springer, Jan; Loeffler, Juergen; Viegas, Carla
    This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings (n = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) (n = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of Mucor sp., Rhizopus sp. or Rhizomucor sp. were not susceptible to 1 mg/L voriconazole, and four isolates of Mucor sp. or Rhizopus sp. were not susceptible to 4 mg/L itraconazole. In conclusion, a combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.
    2019-06Artigo científico Acesso aberto Ver mais
  • Mucorales prevalence and azole-resistance surveillance on different indoor environments: a menace to be tackled
    Publication . Viegas, Carla; Faria, Tiago; Caetano, Liliana Aranha; Loeffler, Juergen; Springer, Jan
    Aim of the study - Mucorales species can cause severe infections among immunocompromised patients. Mucormycosis is associated with a great deal of morbidity and infections caused by Mucorales species among patients receiving triazole prophylaxis. Considering the clinical relevance of Mucorales order, the prevalence of these fungal species and azole-resistance surveillance should be ensured in fungal exposure assessments. In this study, the prevalence of Mucorales species indoor was determined in different occupational settings and dwellings. Azole-resistant Mucorales were determined by morphological and molecular criteria.
    2018-02Documento de conferência Acesso aberto Ver mais