Percorrer por autor "Coelho, Mariana"
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- Anti-hypercholesterolemia effects of edible seaweed extracts and metabolomic changes in Hep-G2 and Caco-2 cell linesPublication . Coelho, Mariana; Pacheco, RitaHypercholesterolemia is a major risk for the development of cardiovascular diseases (CVDs), the main cause of mortality worldwide, and it is characterized by high levels of circulating cholesterol. The drugs currently available for hypercholesterolemia control have several side effects, so it is necessary to develop new effective and safer therapies. Seaweeds serve as sources of several bioactive compounds with claimed beneficial effects. Eisenia bicyclis (Aramé) and Porphyra tenera (Nori) are edible seaweeds that were previously recognized as rich in bioactive compounds. In the present study, we aim to evaluate the anti-hypercholesterolemia effect of these two seaweed extracts and their health potential. Both extracts, but more efficiently Aramé extract, have liver 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) inhibitory activity as well as the capability to reduce approximately 30% of cholesterol permeation through human Caco-2 cells by simulating the intestinal lining, which is a target for hypercholesterolemia treatments. An untargeted metabolomic assay on human intestinal Caco-2 and liver Hep-G2 cell lines exposed to Aramé and Nori extracts revealed changes in the cells’ metabolism, indicating the extracts’ health beneficial effects. The metabolic pathways affected by exposure to both extracts were associated with lipid metabolism, such as phospholipids, and fatty acid metabolism, amino acid pathways, cofactors, vitamins, and cellular respiration metabolism. The effects were more profound in Aramé-treated cells, but they were also observed in Nori-exposed cells. The metabolite modifications were associated with the protection against CVDs and other diseases and to the improvement of the cells’ oxidative stress tolerance. The results obtained for the anti-hypercholesterolemia properties, in addition to the revelation of the positive impact on cell metabolism, offer an important contribution for further evaluation of these seaweed extracts as functional foods or for CVD prevention.
- Edible seaweeds extracts: characterization and functional properties for health conditionsPublication . Coelho, Mariana; Duarte, Ana Patrícia; Pinto, Sofia; Botelho, Hugo M.; Reis, Catarina; Serralheiro, Maria Luisa; Pacheco, RitaSeaweeds are popular foods due to claimed beneficial health effects, but for many there is a lack of scientific evidence. In this study, extracts of the edible seaweeds Arame, Nori, and Fucus are compared. Our approach intends to clarify similarities and differences in the health properties of these seaweeds, thus contributing to target potential applications for each. Additionally, although Arame and Fucus seaweeds are highly explored, information on Nori composition and bioactivities is scarce. The aqueous extracts of the seaweeds were obtained by decoction, then fractionated and characterized according to their composition and biological activity. It was recognized that fractioning the extracts led to bioactivity reduction, suggesting a loss of bioactive compounds synergies. The Arame extract showed the highest antioxidant activity and Nori exhibited the highest potential for acetylcholinesterase inhibition. The identification of the bioactive compounds in the extracts allowed to see that these contained a mixture of phloroglucinol polymers, and it was suggested that Nori's effect on acetylcholinesterase inhibition may be associated with a smaller sized phlorotannins capable of entering the enzyme active site. Overall, these results suggest a promising potential for the use of these seaweed extracts, mainly Arame and Nori, in health improvement and management of diseases, namely those associated to oxidative stress and neurodegeneration.
- Edible seaweeds extracts: characterization and functional properties for health conditionsPublication . Coelho, Mariana; Duarte, Ana Patrícia; Pinto, Sofia; Botelho, Hugo M.; Reis, Catarina Pinto; Serralheiro, Maria Luísa; Pacheco, RitaSeaweeds are popular foods due to claimed beneficial health effects, but for many there is a lack of scientific evidence. In this study, extracts of the edible seaweeds Arame, Nori, and Fucus are compared. Our approach intends to clarify similarities and differences in the health properties of these seaweeds, thus contributing to target potential applications for each. Additionally, although Arame and Fucus seaweeds are highly explored, information on Nori composition and bioactivities is scarce. The aqueous extracts of the seaweeds were obtained by decoction, then fractionated and characterized according to their composition and biological activity. It was recognized that fractioning the extracts led to bioactivity reduction, suggesting a loss of bioactive compounds synergies. The Arame extract showed the highest antioxidant activity and Nori exhibited the highest potential for acetylcholinesterase inhibition. The identification of the bioactive compounds in the extracts allowed to see that these contained a mixture of phloroglucinol polymers, and it was suggested that Nori's effect on acetylcholinesterase inhibition may be associated with a smaller sized phlorotannins capable of entering the enzyme active site. Overall, these results suggest a promising potential for the use of these seaweed extracts, mainly Arame and Nori, in health improvement and management of diseases, namely those associated to oxidative stress and neurodegeneration.
- Perception of mental health in higher education students: changes due to the COVID-19 pandemicPublication . Minghelli, Beatriz; Martins, Beatriz; Pereira, Carlos; Bento, Joana; Nogueira, Leonor; Coelho, Mariana; Pargana, Mariana; Borges, Miriam; Sousa, Rafaela; Calado, TatianaABSTRACT - The actual pandemic context took several changes in the routine of higher education students, including interruptions of face-to-face classes, implementation of education at a distance, interruption or delayed phases, and others, which could compromise mental health. This study aimed to assess the perception of mental health among higher education students in times of the pandemic COVID-19 and associated factors. The sample was constituted of 1,004 higher education students, aged 18 to 51 years (20.33±3.35), 725 (72.2%) female. The instrument includes a questionnaire, elaborated by the researchers, divided into four parts: characterization social-demographic, physical exercise practice, feeding, and mental health. Before the confinement, 99 (9.9%) students classify their mental health as poor and during the confinement 423 (42.2%) said that their mental health was poor (p≤0.001). Women presented 1.91 (CI=1.42-2.56; p≤0.001) more probabilities of having poor mental health and the students that did not practice physical exercise during the confinement had 1.56 (CI=1.22-2.04; p≤0.001) more chances of having poor mental health. Most students classified their mental health as inferior during confinement due to the COVID-19 pandemic, with women and the lack of physical exercise being the factors that were most related to poor mental health.