Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/2833
Título: Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells Kinetic analysis in reverse micelles
Autor: Bernardo, Marisa
Pacheco, Rita
Serralheiro, Maria Luísa M.
Karmali, Amin
Palavras-chave: Amidase
Pseudomonas aeruginosa
Reverse micelles
Hydroxamic acids
Data: Set-2013
Editora: Elsevier Science BV
Citação: BERNARDO, Marisa; PACHECO, Rita; SERRALHEIRO, Maria Luisa M.; KARMALI, Amin - Production of hydroxamic acids by immobilized Pseudomonas aeruginosa cells Kinetic analysis in reverse micelles. Journal of Molecular Catalysis B-Enzymatic. ISSN 1381-1177. Vol. 93 (2013), p. 28-33.
Resumo: Intact cells from Pseudomonas aeruginosa strain L10 containing amidase were used as biocatalysts both free and immobilized in a reverse micellar system. The apparent kinetic constants for the transamidation reaction in hydroxamic acids synthesis, were determined using substrates such as aliphatic, amino acid and aromatic amides and esters, in both media. In reverse micelles, K-m values decreased 2-7 fold relatively to the free biocatalyst using as substrates acetamide, acrylamide, propionamide and glycinamide ethyl ester. We have concluded that overall the affinity of the biocatalyst to each substrate increases when reactions are performed in the reversed micellar system as opposed to the buffer system. The immobilized biocatalyst in general, exhibits higher stability and faster rates of reactions at lower substrates concentration relatively to the free form, which is advantageous. Additionally, the immobilization revealed to be suitable for obtaining the highest yields of hydroxamic acids derivatives, in some cases higher than 80%. (C) 2013 Elsevier B.V. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10400.21/2833
ISSN: 1381-1177
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

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