Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.21/1362
Título: Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis
Autor: Pacheco, Rita
Karmali, Amin Mahamede Vissanji
Serralheiro, Maria Luísa M.
Haris, Parvez I.
Palavras-chave: Recombinant Amidase
Difference FTIR spectroscopy
Substrates interaction
Catalytic mechanism
Transform infrared-spectroscopy
Human serum-albumin
Molecular interpretation
Difference spectra
Data: 2009
Editora: Taylor & Francis LTD
Citação: Pacheco R, Karmali A, Serralheiro M L M, Haris P I. Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis. Biocatalys and Biotransformation. 2009; 27 (5-6): 367-376.
Relatório da Série N.º: 5-6
Resumo: The interaction of a variety of substrates with Pseudomonas aeruginosa native amidase (E.C., overproduced in an Escherichia coli strain, was investigated using difference FTIR spectroscopy. The amides used as substrates showed an increase in hydrogen bonding upon association in multimers, which was not seen with esters. Evidence for an overall reduction or weakening of hydrogen bonding while amide and ester substrates are interacting with the enzyme is presented. The results describe a spectroscopic approach for analysis of substrate-amidase interaction and in situ monitoring of the hydrolysis and transferase reaction when amides or esters are used as substrates.
Peer review: yes
URI: http://hdl.handle.net/10400.21/1362
ISSN: 1024-2422
Aparece nas colecções:ISEL - Eng. Quim. Biol. - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis.rep.pdf51,07 kBAdobe PDFVer/Abrir

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.