Formalin-free histological fixation and its impact on organs, cells, proteins and nucleic acids: a systematic literature review

Lino, Inês; Carneiro, Joana; Viegas, Sara; Borges-Ferro, Amadeu

http://hdl.handle.net/10400.21/17254

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Abstract(s)

To preserve biological tissues and their components against natural degradation, a fixation step must be used providing it with the necessary robustness to withstand further processing. 10% neutral buffered formalin (NBF) is the most widely used fixative, however, this is highly toxic and carcinogenic, posing a risk to the health of professionals and the environment. This review arises from the need to find a less harmful alternative to formalin that could lead to the reduction/elimination of exposure to this reagent. To research the possibility of eliminating the use of formalin, this review aims to compare the results obtained using formalin with alternative formalin-free compounds in biological targets (e.g., organs, cells, proteins, and nucleic acids).

Keywords

Fixation Alternative fixatives Formalin

URI

http://hdl.handle.net/10400.21/17254

Citation

Lino I, Carneiro J, Viegas S, Borges-Ferro A. Formalin-free histological fixation and its impact on organs, cells, proteins and nucleic acids: a systematic literature review. In: II Congresso BioMedLab, Convento de S. Francisco (Coimbra), 1-3 de março de 2024.