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A methodological approach for the simultaneous quantification of glycerol and fatty acids from cork suberin in a single GC run

dc.contributor.authorV Marques, A
dc.contributor.authorPereira, Helena
dc.date.accessioned2019-06-24T08:22:47Z
dc.date.available2019-06-24T08:22:47Z
dc.date.issued2019-11
dc.description.abstractIntroduction: Suberin, as part of plant protective barriers, is one of the most important natural polymers after cellulose and lignin. For a full elucidation of suberin structure the quantification of glycerol, fatty α,ω‐diacids and ω‐hydroxyacids, the major building blocks of suberin, is of primary importance. Glycerol is often lost in the most used analytical procedures or rarely determined by deficient or too laborious techniques. Objectives: Propose a simple, accessible and reliable methanolysis work‐up procedure for an accurate and simultaneous quantification of glycerol and suberin fatty monomers in the same GC run. Material and methods: Cork from Quercus suber L. was depolymerised by methanolysis. Glycerol was derivatised to an organic soluble form before the suberin monomers recovery in water/organic solvent partition. Gas chromatography flame ionisation detector (GC‐FID) response factors were determined for glycerol, ferulic acid and one for each fatty monomer substructure. Additionally, 1,2,4‐butanetriol and methyl nonadecanoate were used as internal standards. Results: The proposed experimental approach allowed the glycerol and all the fatty suberin monomers in the same GC run to be quantified accurately. Glycerol represented 30.6 area%, 14.2 mass% and 38.4 molar% of suberin and the COOH/OH groups ratio was 0.6:1 in the proposed experimental approach in contrast with 0.10 area% and COOH/OH ratio of 3:1 in the most used protocol. Furthermore, ω‐hydroxyacids/α,ω‐diacids mass ratio was 1:1 as opposed to an area ratio of 1.5:1. Conclusion: The proposed work‐up procedure revealed to be a reliable analytical tool for the complete analysis of suberin allowing the future knowledge to grow towards a better understanding of suberin structure throughout its range and variability.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMARQUES, António V.; PEREIRA, Helena – A methodological approach for the simultaneous quantification of glycerol and fatty acids from cork suberin in a single GC run. Phytochemical Analysis. ISSN 1099-1565. Vol. 30, N.º 6 (2019), pp. 687-699pt_PT
dc.identifier.doi10.1002/pca.2846pt_PT
dc.identifier.issn1099-1565
dc.identifier.urihttp://hdl.handle.net/10400.21/10186
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/pca.2846pt_PT
dc.subjectCorkpt_PT
dc.subjectGC‐FIDpt_PT
dc.subjectGlycerolpt_PT
dc.subjectResponse factorspt_PT
dc.subjectSuberinpt_PT
dc.titleA methodological approach for the simultaneous quantification of glycerol and fatty acids from cork suberin in a single GC runpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00239%2F2013/PT
oaire.citation.endPage699pt_PT
oaire.citation.issue6
oaire.citation.startPage687pt_PT
oaire.citation.titlePhytochemical Analysispt_PT
oaire.citation.volume30
oaire.fundingStream5876
person.familyNameMarques
person.familyNamePereira
person.givenNameAntónio
person.givenNameHelena
person.identifier385852
person.identifier.ciencia-idAF1B-8D21-A7AF
person.identifier.orcid0000-0001-7421-6363
person.identifier.orcid0000-0002-5393-4443
person.identifier.scopus-author-id7202134785
person.identifier.scopus-author-id7103251554
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3d0c7ab-dc57-45a9-a86b-6f99a6232f34
relation.isAuthorOfPublication0c88c2d9-25c8-430d-bd54-6f59fce5a354
relation.isAuthorOfPublication.latestForDiscoveryb3d0c7ab-dc57-45a9-a86b-6f99a6232f34
relation.isProjectOfPublication434b74fc-3ebf-4717-b37f-aa3e2084f5d6
relation.isProjectOfPublication.latestForDiscovery434b74fc-3ebf-4717-b37f-aa3e2084f5d6

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