Sousa, Ana CatarinaBaptista, Sara R.Martins, Lígia O.Robalo, Maria Paula2019-01-092019-01-092019-01-04SOUSA, Ana Catarina; [et al] – Synthesis of azobenzene dyes mediated by cotA laccase. Chemistry - an Asian Journal. ISSN 1861-471X. Vol. 14, N.º 1 (2019), pp. 187-1931861-47281861-471Xhttp://hdl.handle.net/10400.21/9292An eco-friendly protocol for the synthesis of azobenzene dyes by oxidative coupling of primary aromatic amines is reported. As efficient biocatalytic systems, CotA laccase and CotA laccase/ABTS (2,2’-azinobis(3-ethylbenzo-thiazoline-6-sulfonic acid)) enablethe oxidation of various substituted anilines, in aqueous medium, ambient atmosphere and under mild reaction conditions of pH and temperature. A series of azobenzene dyes were prepared in good to excellent yields in an one-potreaction. Amechanistic proposal for the formation of the azoderivatives is presented. Our strategy offers an alternative approach for the direct synthesis of azobenzene dyes, avoiding the harsh conditions generally required formost of the traditional synthetic methods.engBiocatalysisDyes/pigmentsGreen chemistryLaccasesOxidative couplingjournal articlehttps://doi.org/10.1002/asia.201801450