Melo, NunoNimtz, ManfredConradt, Harald SFevereiro, Pedro SCosta, Júlia2022-02-022022-02-021997Melo, Nuno S, Nimtz, Manfred, Conradt, Harald S, Fevereiro, Pedro S and Costa, Júlia(1997), Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.), FEBS Letters, 415, doi: 10.1016/S0014-5793(97)01121-60014-5793http://hdl.handle.net/10400.21/14237This paper reports for the first time the presence of the human Lewisa type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxCl from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis.engExtracellular glycoproteinPeroxidasePlant complex oligosaccharideVaccinium myrtillujournal article10.1016/S0014-5793(97)01121-6