Pina-Vaz, CidáliaCosta-de-Oliveira, SofiaRodrigues, Acácio G.Salvador, Alexandre2013-04-232013-04-232004-02Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG, Salvador A. Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples. J Clin Microbiol. 2004;42(2):906-8.0095-1137http://hdl.handle.net/10400.21/2457In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.engTuberculosisMycobacteriaBacilliBacteriaAuraminePropidium iodideCytometryjournal article