Correia, SaraPipio, EvaSantos, RaquelPinto, AndreiaFerro, Amadeu2026-04-172026-04-172026-03Correia S, Pipio E, Santos R, Pinto A, Ferro AB. Contraste em microscopia eletrónica de transmissão: as alternativas emergentes ao acetato de uranilo (revisão sistemática). In: IV Congresso BioMedLab, Centro de Congressos de Aveiro, 6-8 de março de 2026.http://hdl.handle.net/10400.21/22799Transmission electron microscopy (TEM) is a key technique for nanometric ultrastructural analysis in biomedical research. Uranyl acetate (UA) has long been the contrast agent of choice due to its high electron density and affinity for cellular components. However, UA is a radioactive, nephrotoxic, potentially carcinogenic, and subject to strict regulation by the European Chemicals Agency, prompting the need for safer alternatives. This systematic review aimed to evaluate whether non-radioactive substitutes for UA preserve ultrastructural image quality while improving laboratory safety.porMicroscopia eletrónica de transmissãoAcetato de uraniloTransmission electron microscopyUranium compoundsContrast mediaSystematic reviewconference paper