Vaz, FilipaCouto, AndreiaCalado, Cecília2020-07-302020-07-302012-10-18VAZ, Filipa; COUTO, Andreia; CALADO, Cecília R. C. – The importance of Helicobacter pylori's genetic variability for the construction of an efficient vaccine. In 2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG). Coimbra, Portugal: IEEE, 2012. ISBN 978-1-4673-4524-8. Pp. 1-5978-1-4673-4524-8978-1-4673-4526-2http://hdl.handle.net/10400.21/12120Helicobacter pylori is responsible for several gastric diseases. The main constraints of vaccine trials against this pathogen are mainly due to the bacterium high antigenic variability and to down-regulation of the host immune responses. To counteract these factors we propose a DNA vaccine able to induce a balanced humoural and citotoxic specific immune responses, based on multi-antigens. The selection of the antigens NapA, HpaA, VacA and HomB were conducted based on immunoproteomic data and the protein role on infection and pathogenesis. A fragment of each target-antigen was selected by in silico methods based on the maximization of the gene conservation and antigenicity. The set of these small fragments will be presented as a vaccine based on several conserved epitopes of multi-antigenic targets, and consequently representative of the bacterium antigenic variability.engHelicobacter pyloriDNA vaccineMultiantigeniGenetic variabilityConserved epitopesconference object10.1109/ENBENG.2012.6331369