Gomes, Anita QuintalFaria, TiagoCaetano, Liliana AranhaSabino, RaquelViegas, Carla2018-12-282018-12-282018-02Gomes AQ, Faria T, Caetano LA, Sabino R, Viegas C. Analysis of Aspergillus spp. burden by culture based-methods and molecular methods in different occupational environments: what needs to be done? In: 8th Advances Against Aspergillosis, Lisboa Congress Centre (Portugal), 1-3 February 2018.http://hdl.handle.net/10400.21/9212Projeto CalqPCR (IPL/2017/CalqPCR_ESTeSL).Aim of the study - Fungal burden has traditionally been detected by conventional culture analysis, which despite its limitations, is widely used by the scientific community. Alternatively, quantitative real-time PCR (qPCR), based on the amplification of genomic regions specific to certain fungal species, has been associated with increased sensivity, allowing the detection of dormant forms of fungi, such as spores. We present several studies where both methods were used to detect the presence of toxigenic fungi, namely Aspergillus, particularly from the Fumigatus Flavi and Circumdati sections.engOccupational healthOccupational exposureFungiIPL/2017/CalqPCR_ESTeSLconference object