Aranha Caetano, LilianaFaria, TiagoSpringer, JanLoeffler, JuergenViegas, Carla2018-11-272018-11-272019-06Caetano LA, Faria T, Springer J, Loeffler J, Viegas C. Antifungal-resistant mucorales in different indoor environments. Mycology. 2019;10(2):75-83.http://hdl.handle.net/10400.21/9083Project ACT - 005DBB/12, IPL/2016/BBIOR_ESTeSL, IPL/2016/W2E_ESTeSL, 02/SAICT/2016 - Project nº 23222This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings (n = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) (n = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of Mucor sp., Rhizopus sp. or Rhizomucor sp. were not susceptible to 1 mg/L voriconazole, and four isolates of Mucor sp. or Rhizopus sp. were not susceptible to 4 mg/L itraconazole. In conclusion, a combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.engMucoralesAntifungal drug-resistanceIndoor environmentAir-conditioning filterEnvironmental healthPortugalProject nº 23222 (02/SAICT/2016)IPL/2016/W2E_ESTeSLIPL/2016/BBIOR_ESTeSLProject ACT - 005DBB/12journal article10.1080/21501203.2018.1551251