Rodrigues, Ana M.Fale, PedroMadeira, PauloPacheco, RitaFlorêncio, Maria HelenaAscensão, LiaSerralheiro, Maria Luisa2021-01-252021-01-252020-06RODRIGUES, Ana Margarida; [et al] – Phenolic profile and biological activities of decoctions from Santolina impressa, a Portuguese endemic species. Journal of Herbal Medicine. ISSN 2210-8033. Vol. 21 (2020), pp. 1-72210-8033http://hdl.handle.net/10400.21/12679Santolina impressa Hoffmanns & Link is an aromatic Asteraceae species endemic to the southwest of Portugal. It is used in folk medicine as an herbal tea for gastrointestinal ailments and mouthwash antiseptic. The present study aimed to relate the chemical composition of decoctions from the aerial parts S. impressa with their antiacetylcholinesterase, antioxidant, antimicrobial and cytotoxic activities, in an attempt to explain the traditional use of this species. LC–MS/MS analysis identified chlorogenic acid and cynarin as the main components. Antimicrobial activity of the extracts was investigated against two cariogenic bacteria:- Streptococcus sobrinus and Streptococcus mutans, and these decoctions were not cytotoxic towards Caco-2 and HepG2 cell lines. Decoctions from capitula and stems/leaves showed high AChE inhibition, with IC50 values of 328 μg/mL and 579 μg/mL, respectively. The antioxidant activity measured using the DPPH radical scavenging assay showed an EC50 of 14.7 μg/mL and 12.9 μg/mL for capitula and stems/leaves extracts, respectively. The biological activities were kept constant after in vitro digestive process and the HPLC analysis did not indicate changes in the extract’s chemical composition. The results may explain the traditional use of S. impressa decoctions for digestive problems and as a mouthwash antiseptic.engAcetylcholinesterase inhibitionAntioxidant activityCell toxicityCariogenic activityChlorogenic acidCynarinSantolina impressajournal article2210-8041