Brito, MiguelDelgadinho, MarianaGinete, CatarinaMendes, JoanaVasconcelos, J.Santos, Brígida2022-03-162022-03-162020-10Brito M, Delgadinho M, Ginete C, Mendes J, Vasconcelos J, Santos B. Gut microbiota impact on Angolan children with sickle cell disease. In: ASCAT 2020 – The 15th Annual Sickle Cell and Thalassaemia Conference [online], October 26-31, 2020.http://hdl.handle.net/10400.21/14483FCT/Aga Khan (project no. 330842553).FCT_UIDB/05608/2020. FCT_UIDP/05608/2020.Introduction: Clinical manifestations of Sickle cell disease (SCD) are very heterogeneous, and the intestinal microbiome appears to be crucial in the modulation of inflammation, cell adhesion, and induction of aged neutrophils, which are the main interveners of recurrent vaso-occlusive crisis. Enterocyte injury, increased permeability, altered microbial composition, and bacterial overgrowth have all been documented as microbial and pathophysiologic changes in the gut microbiome of SCD patients in recent research studies. Microbiota analysis in SCD populations will be essential to demonstrate the importance of specific bacteria and their function in this disease and provide new insights for attenuating symptoms and new drug targets. Purpose: Given this, our aim is to sequence by NGS bacterial 16S RNA gene in order to characterize the gut microbiome of SCD children and healthy siblings, as a control.engSickle cell diseaseGut microbiomeChildrenAngolaFCT_Aga Khan (project no. 330842553)FCT_UIDB/05608/2020FCT_UIDP/05608/2020conference object